Marileptolyngbya sina gen. nov., sp. nov. and Salileptolyngbya diazotrophicum gen. nov., sp. nov. (Synechococcales, Cyanobacteria), species of cyanobacteria isolated from a marine ecosystem
Marileptolyngbya sina gen. nov., sp. nov. and Salileptolyngbya diazotrophicum gen. nov., sp. nov. (Synechococcales, Cyanobacteria), species of cyanobacteria isolated from a marine ecosystem
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Marileptolyngbya sina gen。
DOI：
10.11646/phytotaxa.383.1.4
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发表时间：
2018-12
期刊：
影响因子：
1.1
通讯作者：
Dong Junde
中科院分区：
文献类型：
--
作者：
Zhou Weiguo;Ding Dewen;Yang Qingsong;Ahmad Manzoor;Zhang Yuanzhou;Lin Xiancheng;Zhang Yanying;Ling Juan;Dong Junde
从海南岛新村湾和南海北方海域分离到两株丝状、非异型囊蓝细菌SCSIO T-2和SCSIO 43686。所研究的菌株的分类位置确定了多相的方法，使用形态，分子和生态分析。这两种蓝藻菌株的细胞大小和鞘的形态不同。SCSIO T-2的16 S rRNA基因测序显示与Leptolyngbya sp. CENA 134（HQ 730083）的相似性为95%，SCSIO 43686显示与Leptolyngbya sp. L21-BG-2（KJ 206339）的相似性为97%。此外，nifH基因序列分析表明，SCSIO T-2与lagerheimii鞘丝藻UTEX 1930（L15550）的同源性为91%，SCSIO 43686与微小纤丝藻乐歌07181（KC 256775）的同源性为88%。这两个菌株的16 S-23 S内转录间隔区（ITS）内的D1-D1 '、Box-B和V3区的二级结构与其密切相关的蓝藻菌株有很大不同。此外，与SCSIO 43686相比，SCSIO T-2菌株显示出相对较高的光利用率，但对光强度的敏感性较低。根据这些特征，将中国海鳞丝藻（Marileptolyngbya sinaSCSIOT-2）命名为中国海鳞丝藻，并建立了一个新属Salileptolyngbya，该属包含一个单系群。我们建议Salileptolyngbya diazotrophicum SCSIO 43686为这个新属的模式种。
We isolated two filamentous, non-heterocystous cyanobacteria, namely SCSIO T-2 from Xincun Bay, Hainan Island and SCSIO 43686 from the northern South China Sea, China. The taxonomic positions of the studied strains were determined by a polyphasic approach that used morphological, molecular, and ecological analyses. The two cyanobacterial strains presented different morphology regarding their cell size and sheaths. Sequencing of the 16S rRNA gene of SCSIO T-2 showed 95% similarity with Leptolyngbya sp. CENA 134 (HQ730083) and SCSIO 43686 displayed 97% similarity with Leptolyngbya sp. L21-BG-2 (KJ206339). In addition, the nifH gene sequence analysis of SCSIO T-2 showed 91% similarity with Lyngbya lagerheimii UTEX 1930 (L15550), while SCSIO 43686 had an 88% similarity with Leptolyngbya minuta LEGE 07181 (KC256775). Both strains’ secondary structures of D1-D1’, Box-B, and V3 regions within the 16S–23S internal transcribed spacer (ITS) region were quite distinct from their closely related cyanobacterial strains. In addition, compared with SCSIO 43686, the SCSIO T-2 strain displayed relatively high light utilization yet low sensitivity to light intensity. Based on these characteristics, the name Marileptolyngbya sina SCSIO T-2 is described, and a new genus named Salileptolyngbya is erected to encompass a monophyletic group. We propose Salileptolyngbya diazotrophicum SCSIO 43686 for the type species of this new genus.