Amelioration of dry eye syndrome in db/db mice with diabetes mellitus by treatment with Tibetan Medicine Formula Jikan Mingmu Drops
Amelioration of dry eye syndrome in db/db mice with diabetes mellitus by treatment with Tibetan Medicine Formula Jikan Mingmu Drops
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藏药方吉坎明目滴剂改善糖尿病db/db小鼠干眼症的研究
DOI：
10.1016/j.jep.2019.111992
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发表时间：
2019-09-15
影响因子：
5.4
通讯作者：
Li, Qi'en
中科院分区：
文献类型：
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作者：
Ai, Xiaopeng;Hou, Ya;Li, Qi'en
民族药理学意义：吉康明目滴剂(JMD)是一种传统藏药，含有六种草药，已被用于治疗糖尿病患者的干眼综合征(DES)。研究目的：然而，JMD改善糖尿病患者的活性此前未见研究。本研究旨在探讨JMD对db/db小鼠的分子机制。材料和方法：采用高效液相色谱和气相色谱-质谱法分析JMD的主要化学成分。然后在db/db小鼠眼表涂抹0.2%苯扎溴铵7天，以诱导DES。分别给予JMD(0.25，0.5，1g/mL)滴眼液或赋形剂滴眼液，每日3次，连续7d，通过酚红线撕裂试验和荧光素钠试验评价疗效。结膜标本行苏木精-伊红染色和高碘酸-席夫染色，观察病理改变和杯状细胞数量。结果：JMD中含有羟基红花黄色素A、木兰花碱、盐酸药根碱、巴马汀、小檗碱、没食子酸、鞣花酸、牛磺熊去氧胆酸、樟脑、异冰片、冰片、反式肉桂酸和麝香酮。JMD治疗组大鼠泪液体积明显增加，角膜荧光素染色积分降低，结膜上皮细胞形态结构得到恢复，结膜组织中IL-6、IL-17α、IL-1β、肿瘤坏死因子-α和血管内皮生长因子水平明显降低。结论：藏药JMD对糖尿病db/db小鼠DES的改善作用可能与其抗炎作用有关。
Ethnopharmacological relevance: Jikan Mingmu Drops (JMD), a traditional Tibetan medicine containing six herbs, has been used to treat dry eye syndrome (DES) in individuals with diabetes mellitus.Aim of study: However, the activity of JMD ameliorates DES with diabetes mellitus has not been previously examined. The aim of the study is to investigate the molecular mechanism of JMD on db/db mice.Materials and methods: The main chemical constituents of JMD were analyzed by high-performance liquid chromatography and gas chromatography-mass spectrometry. DES was then induced in db/db mice by applying 0.2% benzalkonium chloride to the ocular surface for 7 days. Eye drops containing JMD (0.25, 0.5, or 1 g/mL) or vehicle subsequently were administered three times daily for another 7 days, and the therapeutic effects were evaluated by phenol red thread tear and sodium fluorescein tests. Conjunctival specimens were subjected to hematoxylin and eosin staining and periodic acid-Schiff staining to examine pathological changes and number of goblet cells. ELISA was performed to assess the levels of various inflammatory cytokines.Results: JMD contains hydroxysafflor yellow A, magnoflorine, jatrorrhizine hydrochloride, palmatine hydrochloride, berberine hydrochloride, gallic acid, ellagic acid, tauroursodeoxycholic acid, camphor, isoborneol, borneol, trans-cinnamic acid, and muscone. JMD treatment significantly increased the tear volume, decreased the corneal fluorescein staining score, restored the morphology and structure of conjunctival epithelial cells, and markedly downregulated the levels of interleukin (IL)-6, IL-17 alpha, IL-1 beta, tumor necrosis factor-alpha, and vascular endothelial growth factor in the conjunctiva. Further data showed that these protective effects were accompanied by inhibition of inflammation in a dose-dependent manner.Conclusions: Amelioration of DES in db/db mice with diabetes mellitus by treatment with Tibetan medicine formula JMD maybe related to its anti-inflammatory effects.