Apoptotic effects on cultured cells of atmospheric-pressure plasma produced using various gases
Apoptotic effects on cultured cells of atmospheric-pressure plasma produced using various gases
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DOI：
10.7567/jjap.55.01af03
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发表时间：
2016-01-01
影响因子：
1.5
通讯作者：
Kaneko, Toshiro
中科院分区：
文献类型：
--
作者：
Tominami, Kanako;Kanetaka, Hiroyasu;Kaneko, Toshiro
本研究以大鼠成纤维细胞系大鼠成纤维细胞系、大鼠神经母细胞瘤样PC12细胞系和大鼠巨噬细胞样NR8383细胞系为研究对象，研究了低温大气压等离子体对多种细胞的影响。将等离子体直接照射到含有平板细胞的培养液中，培养时间为0-20天，激励电压为3-6千伏，激发频率为10 kHz，气体流量为3 L/min。Annexin-V/碘化丙啶染色检测细胞存活率和细胞凋亡活性。结果表明，低温大气压等离子体辐照以放电电压依赖和照射时间依赖的方式促进细胞死亡。此外，根据细胞类型的不同，会产生不同的效果。此外，氦和氩等离子体诱导细胞凋亡的机制可能完全不同。(C)2016日本应用物理学会
This study investigated the effects of low-temperature atmospheric-pressure plasma on various cells such as rat fibroblastic Rat-1 cell line, rat neuroblastoma-like PC12 cell line, and rat macrophage-like NR8383 cell line. The plasma was irradiated directly to a culture medium containing plated cells for 0-20 s. The applied voltage, excitation frequency, and argon or helium gas flow were, respectively, 3-6 kV, 10 kHz, and 3 L/min. Cell viability and apoptotic activity were evaluated using annexin-V/propidium iodide staining. Results showed that the low-temperature atmospheric-pressure plasma irradiation promoted cell death in a discharge-voltage-dependent and irradiation-time-dependent manner. Furthermore, different effects are produced depending on the cell type. Moreover, entirely different mechanisms might be responsible for the induction of apoptosis in cells by helium and argon plasma. (C) 2016 The Japan Society of Applied Physics