Identification of antibacterial peptides from Maillard reaction products of half-fin anchovy hydrolysates/glucose via LC-ESI-QTOF-MS analysis
Identification of antibacterial peptides from Maillard reaction products of half-fin anchovy hydrolysates/glucose via LC-ESI-QTOF-MS analysis
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DOI：
10.1016/j.jff.2017.07.026
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发表时间：
2017-09
影响因子：
5.6
通讯作者：
Ru Song;Qingqing Shi;Peiyu Yang;Rong-bian Wei
中科院分区：
文献类型：
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作者：
Ru Song;Qingqing Shi;Peiyu Yang;Rong-bian Wei
目的从半鳍金枪鱼水解液/葡萄糖的美拉德反应产物中鉴定抗菌肽。通过液相色谱-电喷雾离子化/多级质谱法鉴定了来自DEAE-Sepharose™ CL-6 B层析和PL aquagel-OH 30 HPLC系统上的P3-2的活性级分的7个肽，并且基于肽序列选择了5个肽用于进一步合成。经Network Protein Sequence @分析和螺旋轮投影预测，阴离子肽FFTQATDLLSR为α-螺旋结构，圆二色性分析进一步证实。在相同浓度下，阳离子肽WLPVVR对大肠杆菌的抑制作用强于其它肽。此外，观察到用肽处理的阴离子1，2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱脂质体囊泡的流体动力学直径的不同尺寸，表明肽在脂质体囊泡上的聚集和分解。据我们所知，这是第一个研究报告，抗菌肽来自半鳍鱼水解物的MRP。
We aimed to identify antibacterial peptides from Maillard reaction products of half-fin anchovy hydrolysates/glucose. Seven peptides from the active fraction of P3-2 derived from DEAE-Sepharose™ CL-6B chromatography and PL aquagel-OH 30 on HPLC system were identified by liquid chromatography-electrospray ionization/multi-stage mass spectrometry, and five peptides were selected for further synthesis on the basis of peptide sequences. The anionic peptide FFTQATDLLSR adopted an α-helical structure, as predicted by Network Protein Sequence @ analysis and helical wheel projection, and further determined using circular dichroism assay. The cationic peptide WLPVVR exhibited stronger inhibition effects againstEscherichia colithan other peptides at similar concentration. Furthermore, differential sizes of hydrodynamic diameters of anionic 1,2-dimyristoyl-sn-glycero-3-phosphocholine liposome vesicles treated with peptides were observed, suggesting the clustering and decomposition of peptide on liposome vesicles. To the best of our knowledge, this is the first study that reports antibacterial peptides derived from the MRPs of half-fin anchovy hydrolysates.