Roles of Pseudomonas aeruginosa Autoinducers and their Degradation Products, Tetramic acids, in Bacterial Survival and Behavior in Ecological Niches
Roles of Pseudomonas aeruginosa Autoinducers and their Degradation Products, Tetramic acids, in Bacterial Survival and Behavior in Ecological Niches
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DOI：
10.1264/jsme2.me10198
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发表时间：
2011-06-01
影响因子：
2.2
通讯作者：
Yamaguchi, Keizo
中科院分区：
文献类型：
--
作者：
Honda, Natsue Hosono;Kimura, Soichiro;Yamaguchi, Keizo
铜绿假单胞菌是一种条件致病菌，主要利用N-酰基高丝氨酸内酯（AHLs）作为自身诱导物。近年来的研究表明，不仅AHLs具有一定的生物活性，其降解产物特特拉姆酸也具有一定的生物活性。本研究探讨了假单胞菌自诱导物和特特拉姆酸在生态位中细菌存活和行为中的作用。化学合成了铜绿假单胞菌自诱导剂及其特特拉姆酸衍生物，并对其构效关系进行了研究。一些来自AHL的特特拉姆酸以浓度依赖性方式（30-300 μ M）引起铜绿假单胞菌活力的显著降低。细菌接种物越小，观察到的杀菌活性越强。特特拉姆酸衍生物的数据表明，特特拉姆酸的酮-烯醇结构对抗菌活性至关重要。外源性特特拉姆酸没有引起生物膜的形成或外产物如绿脓菌素和弹性蛋白酶的产生的显着变化。特胺酸和消毒剂协同作用杀死铜绿假单胞菌。这些结果表明AHL降解产物特特拉姆酸可用于细菌控制。
Pseudomonas aeruginosa, an opportunistic pathogen, is known to mainly use N-acylhomoserine lactones (AHLs) as autoinducers. Recent progress in this field demonstrated that not only AHLs, but also their degradation products, tetramic acids, may have some biological activities. The present study examined the roles of Pseudomonas autoinducers and tetramic acids in bacterial survival and behavior in ecological niches. P. aeruginosa autoinducers and the tetramic acid derivatives were chemically synthesized, and the structure-activity correlation was investigated. Some tetramic acids derived from AHLs caused a significant reduction in the viability of P. aeruginosa in a concentration dependent manner (30-300 mu M). The smaller the inoculum of bacteria, the stronger the bactericidal activity that was observed. The data from tetramic acid derivatives indicated the keto-enol structure of tetramic acid to be critical for the antibacterial activity. Exogenous tetramic acid did not induce significant changes in the formation of biofilm or production of exoproducts such as pyocyanin and elastase. Tetramic acid and disinfectants acted synergistically to kill P. aeruginosa. These results suggest the AHL-degradation product tetramic acid to be useful for bacterial control.