Discovered on gastrointestinal stromal tumour 1 (DOG1): a useful immunohistochemical marker for diagnosing chondroblastoma
Discovered on gastrointestinal stromal tumour 1 (DOG1): a useful immunohistochemical marker for diagnosing chondroblastoma
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DOI：
10.1111/j.1365-2559.2011.04152.x
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发表时间：
2012-06-01
期刊：
影响因子：
6.4
通讯作者：
Zustin, Jozef
中科院分区：
文献类型：
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作者：
Akpalo, Hana;Lange, Claudia;Zustin, Jozef
Akpalo H，Lange C & Zustin J？（2012）Histopiology similar to 60，10991106在胃肠道间质瘤1（DOG 1）上发现：用于诊断成软骨细胞瘤的有用免疫组织化学标记物目的：成软骨细胞瘤的细胞区域由具有锯齿状核的多边形成软骨细胞和分散的破骨细胞型多核细胞组成。为了进一步了解成软骨细胞的表型，我们研究了几种已建立的免疫组化标记物在成软骨细胞瘤中的表达。方法与结果：使用免疫组织化学抗体[CD 34、α-平滑肌肌动蛋白（α-SMA）、DOG 1、CD 117、AE 1/AE 3和CD 163]分析了9例软骨母细胞瘤。十软骨粘液样纤维瘤，七骨巨细胞瘤和四个胎儿股骨近端进行了分析。每个成软骨细胞瘤的细胞区域含有DOG 1 +aSMA+ CD 117-CD 34-成软骨细胞的巢，这是在软骨粘液样纤维瘤病例或巨细胞瘤中未检测到的表型。虽然AE 1/AE 3在所有软骨母细胞瘤中均有表达，但染色强度和阳性细胞比例差异很大。在软骨母细胞瘤、软骨粘液样纤维瘤和巨细胞瘤的所有病例中均检测到病变内CD 163+巨噬细胞。结论：我们的研究结果表明，膜DOG 1+成软骨细胞巢位于成软骨细胞瘤的细胞部分内，含有弥漫性异质性浸润，主要是DOG 1-成软骨细胞，CD 163+巨噬细胞和多核骨巨细胞。因此，软骨母细胞瘤可以添加到通常为DOG 1阳性的肿瘤，以及胃肠道间质瘤（GIST），胰腺罕见的实性假乳头状肿瘤和特殊的间质肿瘤，包括子宫型腹膜后平滑肌瘤，腹膜平滑肌瘤病和滑膜肉瘤。
Akpalo H, Lange C & Zustin J ?(2012) Histopathology similar to 60, 10991106 Discovered on gastrointestinal stromal tumour 1 (DOG1): a useful immunohistochemical marker for diagnosing chondroblastoma Aims: Cellular areas of chondroblastoma are composed of polygonal chondroblasts with indented nuclei and scattered osteoclast-type multinucleated cells. To learn more about the phenotype of chondroblasts, we investigated the expression of several established immunohistochemical markers in chondroblastomas. Methods and results: Nine chondroblastomas were analysed using immunohistochemical antibodies [CD34, a-smooth muscle actin (a-SMA), DOG1, CD117, AE1/AE3 and CD163]. Ten chondromyxoid fibromas, seven giant cell tumours of bone and four foetal proximal femurs were also analysed. The cellular areas of each chondroblastoma contained nests of DOG1+aSMA+ CD117- CD34- chondroblasts, a phenotype that was not detected in chondromyxoid fibroma cases or in giant cell tumours. Although AE1/AE3 was expressed in all chondroblastomas, the staining intensity and proportion of the positive cells varied widely. Intra-lesional CD163+ macrophages were detected in all cases of chondroblastoma, chondromyxoid fibroma and giant cell tumours. Conclusions: Our results demonstrated nests of membranous DOG1+ chondroblasts located within cellular portions of chondroblastoma containing diffuse heterogeneous infiltrates of mostly DOG1- chondroblasts, CD163+ macrophages and multinucleated osteoclastic giant cells. Thus, chondroblastoma can be added to the tumours that are usually positive for DOG1, alongside gastrointestinal stromal tumour (GIST), rare solid-pseudopapillary neoplasms of the pancreas and exceptional mesenchymal tumours including uterine type retroperitoneal leiomyoma, peritoneal leiomyomatosis and synovial sarcoma.