Regulatory role of UvrY in adaptation of Photorhabdus luminescens growth inside the insect
Regulatory role of UvrY in adaptation of Photorhabdus luminescens growth inside the insect
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DOI：
10.1111/j.1462-2920.2007.01528.x
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发表时间：
2008-05-01
影响因子：
5.1
通讯作者：
Danchin, Antoine
中科院分区：
文献类型：
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作者：
Krin, Evelyne;Derzelle, Sylviane;Danchin, Antoine
我们报告了一个uvrY缺陷的发光杆菌突变体的全球表达谱。我们发现，两组分调控系统BarA/UvrY的调节剂部分调节超过500个靶基因，这些靶基因编码参与细胞主要隔室和代谢途径合成的功能。这种调节似乎是部分间接的，因为UvrY影响了几种调节剂的表达。事实上，鞭毛生物合成转录激活因子FlhC和鞭毛调节子在不存在UvrY的情况下被诱导，导致超鞭毛表型和运动性和生物膜形成的增加。两个主要的调节系统也被改变：2型群体感应诱导剂AI-2被激活UvrY，和CsrA调节功能似乎被抑制的小非翻译RNA的csrB，CsrA活性抑制剂TldD和伴侣GroESL的增加。通过和独立于这些系统，UvrY调节氧化应激抗性;生物发光;铁，糖和肽转运;蛋白酶;聚酮合成酶和核碱基再循环，与昆虫降解和细菌同化有关。因此，uvrY缺陷型菌株表现出对昆虫细胞的杀伤减少和在昆虫细胞培养物上的生长减少，这表明uvrY在发光拟青霉在昆虫内部的适应中起作用。
We report global expression profiling of a uvrY-deficient mutant of Photorhabdus luminescens. We found that the regulator moiety of the two-component regulatory system BarA/UvrY regulated more than 500 target genes coding for functions involved in the synthesis of major compartments and metabolic pathways of the cell. This regulation appeared to be in part indirect as UvrY affected the expression of several regulators. Indeed, the flagellum biosynthesis transcription activator FlhC and the flagella regulon were induced in the absence of UvrY, leading to a hyperflagellated phenotype and an increase in motility and biofilm formation. Two major regulatory systems were also altered: the type 2 quorum-sensing inducer AI-2 was activated by UvrY, and the CsrA regulator function appeared to be repressed by the increase of the small-untranslated RNA csrB, the CsrA activity inhibitor TldD and the chaperonin GroESL. Both through and independently of these systems, UvrY regulated oxidative stress resistance; bioluminescence; iron, sugar and peptide transport; proteases; polyketide synthesis enzymes and nucleobases recycling, related to insect degradation and assimilation by bacteria. As a consequence, the uvrY-deficient strain exhibited a decreased killing of insect cells and a reduced growth on insect cells culture, suggesting a UvrY role in the adaptation of P. luminescens inside the insect.