A controlled release system of titanocene dichloride by electrospun fiber and its antitumor activity in vitro
A controlled release system of titanocene dichloride by electrospun fiber and its antitumor activity in vitro
复制标题
静电纺纤维二氯化二茂钛控释系统及其体外抗肿瘤活性
DOI：
10.1016/j.ejpb.2010.09.005
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发表时间：
2010-11-01
影响因子：
4.9
通讯作者：
Hue, Wen
中科院分区：
文献类型：
--
作者：
Chen, Ping;Wu, Qing-Sheng;Hue, Wen
为了提高二氯二茂钛化疗药物的安全性和有效性，克服二氯二茂钛在人体内不稳定、半衰期短等缺点，我们报道了一种二氯二茂钛的静电纺丝纤维控释系统及其对人肺癌spca-1细胞的体外抗肿瘤活性。该系统是通过静电纺丝。采用紫外-可见分光光度计研究了二氯二茂钛在PBS中的释放行为。用MTT法检测了纤维的体外抗肿瘤活性。二氯二茂钛可很好地掺入到可生物降解的聚L-乳酸纤维中。XRD结果表明，二氯二茂钛在纤维中以无定形形式存在。二氯二茂钛可实现长时间的缓释。MTT法测得纤维毡中二氯二茂钛的实际含量为40、80、160和240 mg/L时，细胞生长抑制率分别为11.2%、22.1%、44.2%和68.2%。释放的二氯化二茂钛对肺肿瘤细胞有明显的抑制作用。该系统具有控制二氯二茂钛释放的作用，有望作为一种可植入的抗癌药物在临床上应用。(C)2010 Elsevier B. V.保留所有权利。
In order to improve both safety and efficacy of cancer chemotherapy of titanocene dichloride and overcome the shortcomings such as instability and short half-life in the human body, we report a controlled release system of titanocene dichloride by electrospun fiber and its in vitro antitumor activity against human lung tumor spca-1 cells. The system was developed by electrospinning. The release profiles of titanocene dichloride in PBS were researched by UV-Vis spectrophotometer. In vitro antitumor activities of the fibers were examined by MTT method. Titanocene dichloride was well incorporated in biodegradable poly(L-lactic acid) fibers. XRD results suggest that titanocene dichloride exists in the amorphous form in the fibers. The controlled release of titanocene dichloride can be gained for long time. MTT showed actual titanocene dichloride content 40, 80, 160 and 240 mg/L from the fibers mat, cell growth inhibition rates of 11.2%, 22.1%, 44.2% and 68.2% were achieved, respectively. The titanocene dichloride released has obvious inhibition effect against lung tumor cells. The system has an effect of controlled release of titanocene dichloride and may be used as an implantable anticancer drug in clinical applications in the future. (C) 2010 Elsevier B.V. All rights reserved.