Expression of neuropeptide Y and gonadotropin-releasing hormone gene types in the brain of female Nile tilapia (Oreochromis niloticus) during mouthbrooding and food restriction
Expression of neuropeptide Y and gonadotropin-releasing hormone gene types in the brain of female Nile tilapia (Oreochromis niloticus) during mouthbrooding and food restriction
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DOI：
10.1016/j.peptides.2018.10.009
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发表时间：
2019-02-01
期刊：
影响因子：
3
通讯作者：
Parhar, Ishwar S.
中科院分区：
文献类型：
--
作者：
Das, Kalpana;Ogawa, Satoshi;Parhar, Ishwar S.
尼罗罗非鱼（Oreochromis niloticus）是一种丽鱼，是一种母鱼，在产卵期间能量消耗最小，卵巢周期较慢。本研究的目的是观察在育口期（4天和12天）、限食期（12天和12天）和限食后再喂养期（12天）参与食欲和生殖控制的关键神经肽（神经肽Ya (NPYa)）、生殖神经肽：促性腺激素释放激素（GnRH1、GnRH2和GnRH3）和接吻素（kisspeptin）基因表达的变化。食物限制显著增加了端脑npya mRNA水平。然而，npya mRNA水平在育口期间没有显著变化。与限食雌性相比，口育雌性gnrh1 mRNA水平显著降低。与对照组相比，经口育12天、限食12天、复食12天的雌性gnrh3 mRNA水平也显著降低。不同饲养方式组间gnrh2和kiss2 mRNA水平无显著差异。外周代谢信号分子ghrelin （GHS-Rl a和GHS-Rlb）和leptin （lepp - r）受体mRNA水平未见显著变化。这些结果表明，端脑npya mRNA水平不受影响可能有助于抑制口育雌性罗非鱼的食欲。此外，gnrh1和gnrh3 mRNA水平的降低可能会影响卵巢周期的进展和生殖行为等生殖功能的抑制，而GnRH2和Kiss2在限食条件下的生殖中可能没有显著作用。
A cichlid fish, the Nile tilapia (Oreochromis niloticus), is a maternal mouthbrooder, which exhibits minimum energy expenditure and slower ovarian cycles during mouthbrooding. The objective of this study was to observe changes in the gene expression of key neuropeptides involved in the control of appetite and reproduction, including neuropeptide Y a (NPYa), reproductive neuropeptides: gonadotropin-releasing hormone (GnRH1, GnRH2 and GnRH3) and kisspeptin (Kiss2) during mouthbrooding (4- and 12-days), 12-days of food restriction and 12-days of food restriction followed by refeeding. The food restriction regime showed a significant increase in npya mRNA levels in the telencephalon. However, there were no significant alterations in npya mRNA levels during mouthbrooding. gnrh1 mRNA levels were significantly lower in mouthbrooding female as compared with females with food restriction. gnrh3 mRNA levels were also significantly lower in female with 12-days of mouthbrooding, 12-days of food restriction followed by 12-days of refeeding when compared with controls. There were no significant differences in gnrh2 and kiss2 mRNA levels between groups under different feeding regimes. No significant changes were observed in mRNA levels of receptors for peripheral metabolic signaling molecules: ghrelin (GHS-Rl a and GHS-Rlb) and leptin (Lep-R). These results suggested that unaffected npya mRNA levels in the telencephalon might contribute to suppression of appetite in mouthbrooding female tilapia. Furthermore, lower gnrh1 and gnrh3 mRNA levels may influence the suppression of reproductive functions such as progression of ovarian cycle and reproductive behaviours, while GnRH2 and Kiss2 may not play a significant roles in reproduction under food restriction condition.