Design methodology for a confocal imaging system using an objective microlens array with an increased working distance.
Design methodology for a confocal imaging system using an objective microlens array with an increased working distance.
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DOI：
10.1038/srep33278
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发表时间：
2016-09-12
影响因子：
4.6
通讯作者：
Kang S
中科院分区：
文献类型：
--
作者：
Choi W;Shin R;Lim J;Kang S
在这项研究中，多光学探头共焦成像系统的设计方法的发展。为了开发具有所需分辨率和成像区域的成像系统，本研究着重于设计方法，以创建可扩展且易于实现的共焦成像系统。该系统采用由两个微透镜阵列组成的微物镜透镜模块和远心中继光学系统，克服了传统多光探针共焦成像系统光学复杂、工作距离短的局限性。微物镜透镜模块采用背面对准光刻和热回流工艺制作在玻璃基板上。为了验证该方法的可行性，设计并构建了一个分辨率为1 μm/pixel的光学系统，该系统使用了10 × 10的多个光学探针阵列。该系统视场为1 mm × 1 mm，样品扫描范围为100 μm。通过使用刀口检测方法进行样品测试来评价光学分辨率。系统的横向分辨率为0.98 μm。
In this study, a design methodology for a multi-optical probe confocal imaging system was developed. To develop an imaging system that has the required resolving power and imaging area, this study focused on a design methodology to create a scalable and easy-to-implement confocal imaging system. This system overcomes the limitations of the optical complexities of conventional multi-optical probe confocal imaging systems and the short working distance using a micro-objective lens module composed of two microlens arrays and a telecentric relay optical system. The micro-objective lens module was fabricated on a glass substrate using backside alignment photolithography and thermal reflow processes. To test the feasibility of the developed methodology, an optical system with a resolution of 1 μm/pixel using multi-optical probes with an array size of 10 × 10 was designed and constructed. The developed system provides a 1 mm × 1 mm field of view and a sample scanning range of 100 μm. The optical resolution was evaluated by conducting sample tests using a knife-edge detecting method. The measured lateral resolution of the system was 0.98 μm.