Microbial Co-Occurrence in Floral Nectar Affects Metabolites and Attractiveness to a Generalist Pollinator
Microbial Co-Occurrence in Floral Nectar Affects Metabolites and Attractiveness to a Generalist Pollinator
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DOI：
10.1007/s10886-020-01169-3
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发表时间：
2020-04
影响因子：
2.3
通讯作者：
Caitlin C. Rering;R. Vannette;R. Schaeffer;J. Beck
中科院分区：
文献类型：
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作者：
Caitlin C. Rering;R. Vannette;R. Schaeffer;J. Beck
微生物代谢可以在服务资源共生中形成对动物吸引力重要的线索。资源经常被微生物群落定植，但动物-微生物相互作用的实验评估往往集中在微生物单一培养。这种方法可能无法预测微生物组合的影响，因为微生物-微生物相互作用可能以非加性的方式影响微生物代谢和由此产生的化学感觉线索。在这里，我们比较了微生物单培养和共培养对组成微生物生长、挥发性代谢物产生、糖分解代谢的影响，以及在两种不同糖浓度的花蜜环境中对传粉者觅食的影响。在共培养中，酵母菌的丰度下降，但细菌的丰度没有下降。不同微生物处理和花蜜浓度的挥发物排放差异显著，而单株和共培养的糖浓度相对相似。共培养挥发物排放近似于单一培养挥发物的加性组合。尽管微生物生长和化学感觉线索存在差异，但蜜蜂的摄食在微生物单一培养和组合之间没有差异。综上所示，我们的研究结果表明，在某些情况下，微生物组合的化学和生态效应在很大程度上可以从组分物种的化学和生态效应中预测出来，但要预测在什么情况下非加性效应是重要的，还需要做更多的工作。
Microbial metabolism can shape cues important for animal attraction in service-resource mutualisms. Resources are frequently colonized by microbial communities, but experimental assessment of animal-microbial interactions often focus on microbial monocultures. Such an approach likely fails to predict effects of microbial assemblages, as microbe-microbe interactions may affect in a non-additive manner microbial metabolism and resulting chemosensory cues. Here, we compared effects of microbial mono- and cocultures on growth of constituent microbes, volatile metabolite production, sugar catabolism, and effects on pollinator foraging across two nectar environments that differed in sugar concentration. Growth in co-culture decreased the abundance of the yeastMetschnikowia reukaufii, but not the bacteriumAsaia astilbes. Volatile emissions differed significantly between microbial treatments and with nectar concentration, while sugar concentration was relatively similar among mono- and cocultures. Coculture volatile emission closely resembled an additive combination of monoculture volatiles. Despite differences in microbial growth and chemosensory cues, honey bee feeding did not differ between microbial monocultures and assemblages. Taken together, our results suggest that in some cases, chemical and ecological effects of microbial assemblages are largely predictable from those of component species, but caution that more work is necessary to predict under what circumstances non-additive effects are important.