INVESTIGATION OF A NEW RENAL TUMOR MODEL
INVESTIGATION OF A NEW RENAL TUMOR MODEL
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DOI：
10.1016/0022-4804(73)90096-6
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发表时间：
1973-01-01
影响因子：
2.2
通讯作者：
MURPHY, GP
中科院分区：
文献类型：
--
作者：
HRUSHESKY, WJ;MURPHY, GP
材料与方法本研究的肿瘤是在近交Balb C/Cr小鼠中自发产生的肾腺癌。这项研究是在肿瘤转移到第六代时开始的。肿瘤通过单细胞悬浮液转移。在无菌玻璃组织研磨机中研磨1 g切好的肿瘤块，加入9 ml冷冻无菌4℃McCoy培养基（5%胎牛血清，青霉素100单位/ml，链霉素50 pg/ml）制成混悬液。溶液通过27号针头，通过红血素染色[9]测定活力。生存力始终在90%到95%之间。然后将浓度调整为每毫升1 × 10”活肿瘤细胞。动物接受总标准剂量为。1 ml或1 × 10”活肿瘤细胞，按一或两等分，除非另有说明。所有肿瘤接种均使用27guagc针头和无菌11，$ cc注射器。悬浮液保持在4℃，并经常搅拌，以防止沉淀出细胞。
MATERIALS AND METHODSThe tumor being investigated arose spontaneously as a renal adenocarcinoma in an inbred Balb C/Cr mouse. The study was begun when the tumor was in its sixth transfer generation. The tumor is transferred by a single cell suspension. The suspension is made by grinding 1 g of trimrned diced tumor in a sterile glass tissue grinder with 9 ml of sterile chilled 4 C McCoy’s medium (5% fetal bovine sera, penicillin, 100 units/ml, and streptomycin 50 pg/ml). The solution is passed through a 27-gauge needle and viability is determined by erythrocin staining[9]. The viability ranges consistently from 90 to> 95%. The concentration is then adjusted to 1 X 10” live tumor cells per ml. The animals receive a total standard dose of. l ml or 1 X 10” live tumor cells in one or two aliquots unless otherwise indicated. A 27-guagc needle and sterile I, $ cc cyringe are used for all tumor inoculations. The suspension is kept at 4 C and agitated frequently to prevent settling out of cells.