Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques:: A high-throughput nano-LC-IMS-TOF separation of peptides arising from a Drosophila protein extract
Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques:: A high-throughput nano-LC-IMS-TOF separation of peptides arising from a Drosophila protein extract
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DOI：
10.1021/ac030107f
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发表时间：
2003-10-01
影响因子：
7.4
通讯作者：
Clemmer, DE
中科院分区：
文献类型：
--
作者：
Myung, S;Lee, YJ;Clemmer, DE
已开发出用于离子淌度飞行时间质谱仪的线性八极阱接口，用于聚焦和累积电喷雾电离产生的连续离子束。与利用三维 Paul 几何陷阱的类似配置相比，该接口将实验效率提高了 50-200 倍（Hoaglund-Hyzer, C. S.；Lee, Y. J.；Counterman, A. E.；Clemmer, D. E. Anal. Chem. 2002, 74, 992-1006）。通过这些改进，可以在几分之一秒内记录复杂混合物的嵌套漂移（飞行）时间分布。我们展示了几种明确的肽混合物的方法，并给出了检测限的评估。此外，我们通过在线三维纳米 LC 离子淌度 TOF 分离果蝇蛋白质组中的胰蛋白酶肽，展示了该方法在蛋白质组学领域的实用性。
A linear octopole trap interface for an ion mobility time-of-flight mass spectrometer has been developed for focusing and accumulating continuous beams of ions produced by electrospray ionization. The interface improves experimental efficiencies by factors of similar to50-200 compared with an analogous configuration that utilizes a three-dimensional Paul geometry trap (Hoaglund-Hyzer, C. S.; Lee, Y. J.; Counterman, A. E.; Clemmer, D. E. Anal. Chem. 2002, 74, 992-1006). With these improvements, it is possible to record nested drift (flight) time distributions for complex mixtures in fractions of a second. We demonstrate the approach for several well-defined peptide mixtures and an assessment of the detection limits is given. Additionally, we demonstrate the utility of the approach in the field of proteomics by an on-line, three-dimensional nano-LC-ion mobility-TOF separation of tryptic peptides from the Drosophila proteome.