Engineered catalytic biofilms for continuous large scale production of n-octanol and (S)-styrene oxide
Engineered catalytic biofilms for continuous large scale production of n-octanol and (S)-styrene oxide
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DOI：
10.1002/bit.24629
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发表时间：
2013-02-01
影响因子：
3.8
通讯作者：
Schmid, Andreas
中科院分区：
文献类型：
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作者：
Gross, Rainer;Buehler, Katja;Schmid, Andreas
本研究通过理论上设计一种采用膜纤维组件的工艺，并将相应的工艺参数与经典的搅拌槽研究进行比较，来评估基于生物膜的生物转化在工业规模上的技术可行性。据我们所知，用于精细化学品生产的催化生物膜工艺迄今尚未在技术规模上报道。作为模型反应，我们采用了以前研究的不对称苯乙烯环氧化假单胞菌菌株VLB 120？C生物膜和此处描述的选择性烷烃羟基化。利用重组恶臭假单胞菌PpS 81 pBT 10生物膜中的恶臭假单胞菌Gpo 1的非血红素含铁烷烃羟化酶系统（AlkBGT），我们能够连续地从辛烷生产1-辛醇，最大生产率为1.3？g的？我？aq-1？在单管微反应器中进行。为了可能的工业应用，提出了一种填充有84，000根聚丙烯纤维的圆柱形膜纤维组件。根据这里提出的计算，59个膜纤维组件（0.9？m直径和2？m长）将是可行的，以实现1,000？氧化苯乙烯吨/年。此外，与传统的搅拌罐反应器工艺相比，碳的产物产率至少可以增加一倍，并且产生的生物质废物将减少400倍以上。相反，对于辛醇工艺，需要进一步强化生物活性和/或扩大表面膜以达到生产规模。通过考虑生物质生长控制和保持恒定的生物活性等挑战，这项研究表明，在工业规模上生产精细化学品的生物膜工艺在产品产量和生物质废物生产方面是一种可持续的替代方案。Biotechnol. Bioeng. 2013; 110：424436. (c)2012年威利期刊公司
This study evaluates the technical feasibility of biofilm-based biotransformations at an industrial scale by theoretically designing a process employing membrane fiber modules as being used in the chemical industry and compares the respective process parameters to classical stirred-tank studies. To our knowledge, catalytic biofilm processes for fine chemicals production have so far not been reported on a technical scale. As model reactions, we applied the previously studied asymmetric styrene epoxidation employing Pseudomonas sp. strain VLB120?C biofilms and the here-described selective alkane hydroxylation. Using the non-heme iron containing alkane hydroxylase system (AlkBGT) from P. putida Gpo1 in the recombinant P. putida PpS81 pBT10 biofilm, we were able to continuously produce 1-octanol from octane with a maximal productivity of 1.3?g?L?aq-1?day-1 in a single tube micro reactor. For a possible industrial application, a cylindrical membrane fiber module packed with 84,000 polypropylene fibers is proposed. Based on the here presented calculations, 59 membrane fiber modules (of 0.9?m diameter and 2?m length) would be feasible to realize a production process of 1,000?tons/year for styrene oxide. Moreover, the product yield on carbon can at least be doubled and over 400-fold less biomass waste would be generated compared to classical stirred-tank reactor processes. For the octanol process, instead, further intensification in biological activity and/or surface membrane enlargement is required to reach production scale. By taking into consideration challenges such as biomass growth control and maintaining a constant biological activity, this study shows that a biofilm process at an industrial scale for the production of fine chemicals is a sustainable alternative in terms of product yield and biomass waste production. Biotechnol. Bioeng. 2013; 110: 424436. (c) 2012 Wiley Periodicals, Inc.