Targeting of proteins to endoplasmic reticulum-derived compartments in plants. The importance of RNA localization
Targeting of proteins to endoplasmic reticulum-derived compartments in plants. The importance of RNA localization
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DOI：
10.1104/pp.104.048934
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发表时间：
2004-11-01
期刊：
影响因子：
7.4
通讯作者：
Satoh, H
中科院分区：
文献类型：
--
作者：
Crofts, AJ;Washida, H;Satoh, H
将蛋白质靶向内质网(ER)是一个相当感兴趣的话题，因为这个细胞器是蛋白质进入其他细胞器以及内质网本身的入口。植物的一个独特特征是，它们能够在内质网中储存蛋白质，而这种储存蛋白质的沉积为人类和动物提供了重要的营养来源。因此，增加我们对将贮藏蛋白质定向到这个关键细胞器所需机制的了解，最终将允许修改关键步骤，导致植物作为蛋白质来源以及作物产量和生产力的改善。蛋白质进入内膜系统依赖于瞬时N-末端信号肽的存在。然而，最近的发展表明，RNA定位于特定的ER亚区可能有助于蛋白质在膜内系统的靶向。这一更新的目的是引入RNA定位作为一种将蛋白质合成定位于特定细胞内位置的方法，重点是将醇溶蛋白mRNA定位于被称为蛋白体的内质网衍生的隔室中的蛋白质。除了总结已发表的关于醇溶蛋白mRNA定位的研究外，还将讨论未发表的数据，这些数据表明存在通往特定ER亚区的多条RNA定位途径，以及mRNA靶向在蛋白质定位方面的作用。
The targeting of proteins to the endoplasmic reticulum (ER) is a topic of considerable interest since this organelle serves as an entry point for proteins destined for other organelles, as well as for the ER itself. A unique feature of plants is that they are able to store proteins in the ER in addition to other endomembrane compartments, and the deposition of such storage proteins provides important sources of both human and animal nutrition. Thus, increasing our knowledge of the mechanisms required for the targeting of storage proteins to this crucial organelle will ultimately allow the modification of critical steps, leading to improvements in plants as a protein source as well as in crop yield and productivity. The entry of proteins into the endomembrane system is dependent on the presence of a transient N-terminal signal peptide. However, recent developments indicate that the localization of RNAs to specific ER subdomains may facilitate protein targeting within the endomembrane system. The aim of this Update is to introduce RNA localization as a means of targeting protein synthesis to specific intracellular locations with a focus on the localization of prolamine mRNA, and thus protein, to the ER-derived compartment known as the protein body. In addition to summarizing published research concerning prolamine mRNA localization, unpublished data showing the existence of multiple RNA localization pathways to specific ER subdomains and the role of mRNA targeting with respect to protein localization will be discussed.