BARIUM SUPPRESSES SLOW PIII IN PERFUSED BULLFROG RETINA
BARIUM SUPPRESSES SLOW PIII IN PERFUSED BULLFROG RETINA
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DOI：
10.1016/0042-6989(79)90006-3
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发表时间：
1979-01-01
期刊：
影响因子：
1.8
通讯作者：
SILLMAN, AJ
中科院分区：
文献类型：
--
作者：
BOLNICK, DA;WALTER, AE;SILLMAN, AJ
PIII [一个缓慢的，大的，负的电位变化]光刺激的脊椎动物进行了研究，使用Ba。光感受器附近K降低可引起Mueller细胞[MC]过度增殖并引起PIII反应。Ba降低K电导，提供MC可能的PIII响应的测试。将暗适应的牛蛙视网膜与色素上皮分离，并用含有Tris苹果酸的缓冲液灌注。对光刺激[250 ms，47 μ W/cm 2]的响应的细胞外记录通过在正常缓冲液条件下和在Ba Ringers溶液[0.4 mM BaCl 2]中嵌入灌注室中的ClAg电极进行。Ba溶液抑制慢PIII响应。在返回到正常溶液后，PIII反应缓慢返回[115分钟部分返回]，与在20-30分钟内完全返回的光感受器杆和锥反应形成对比。缓慢的PIII反应显然发生在MC中。
PIII [a slow, large, negative potential change] response to light stimulus in the vertebrate was studied using Ba. K decrease near the photoreceptor could cause Mueller cells [MC] to hyperpolarize and elicit a PIII response. Ba decreases K conductance providing a test of the MC possible PIII response. Dark adapted bullfrog retina were separated from the pigment epithelium and perfused with Ringers solution buffered with Tris malate. Extracellular recordings of response to light stimulus [250 ms, 47 .mu.W/cm2] were made by ClAg electrodes embedded in the perfusion chamber under normal buffer conditions and in Ba Ringers solution [0.4 mM BaCl2]. The Ba solution suppressed the slow PIII response. After return to the normal solution the PIII response returned slowly [115 min partial return] contrasting to the photoreceptors rod and cone responses that returned fully within 20-30 min. The slow PIII response apparently occurs in the MC.