Label-Free Biosensor Arrays Based on Silicon Ring Resonators and High-Speed Optical Scanning Instrumentation
Label-Free Biosensor Arrays Based on Silicon Ring Resonators and High-Speed Optical Scanning Instrumentation
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DOI：
10.1109/jstqe.2009.2032510
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发表时间：
2010-05-01
影响因子：
4.9
通讯作者：
Gunn, L. Cary
中科院分区：
文献类型：
--
作者：
Iqbal, Muzammil;Gleeson, Martin A.;Gunn, L. Cary
本文提出了一种基于硅环谐振器的传感器阵列快速实时结合分析平台。一个由32个传感器组成的阵列同时被询问。采用8个传感器作为控制，同时产生24条结合曲线。该系统的体折射率灵敏度可达7.6 x 10(-7)，传感器间变异性为3.9%。在8分钟的孵育过程中，使用固定化生物素捕获稀释在牛血清白蛋白溶液中的链霉亲和素，观察到在8 log浓度下至60 fM的实时结合。用两个DNA寡核苷酸探针证明了复合介质中的多路复用。结果的时间和可重复性证明是足够的临床应用。
A platform for performing rapid, real-time binding assays on sensor arrays based on silicon ring resonators is presented in this paper. An array of 32 sensors is interrogated simultaneously. Using eight sensors as controls, 24 simultaneous binding curves are produced. The bulk refractive index sensitivity of the system was demonstrated down to 7.6 x 10(-7) and sensor-to-sensor variability is 3.9%. Using an 8-min incubation, real-time binding was observed over 8-logs of concentration down to 60 fM using immobilized biotin to capture streptavidin diluted in bovine serum albumin solution. Multiplexing in complex media is demonstrated with two DNA oligonucleotide probes. Time to result and repeatability are demonstrated to be adequate for clinical applications.