The nitrate assimilation pathway is involved in the trap formation of Arthrobotrys oligospora, a nematode-trapping fungus
The nitrate assimilation pathway is involved in the trap formation of Arthrobotrys oligospora, a nematode-trapping fungus
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硝酸盐同化途径参与寡孢节孢菌（一种线虫捕获真菌）的捕获形成
DOI：
10.1016/j.fgb.2016.05.003
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发表时间：
2016-07-01
影响因子：
3
通讯作者：
Zhang, Ke-Qin
中科院分区：
文献类型：
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作者：
Liang, Lianming;Liu, Zhiheng;Zhang, Ke-Qin
营养条件对于陷阱的形成以及捕食线虫真菌从腐生生活方式向捕食生活方式的转变非常重要。少孢节孢菌是一种典型的捕食线虫的真菌，它可以通过捕食线虫而形成陷阱来捕食和杀死线虫。营养有限的环境以前被证明是重要的陷阱的形成，这是由几个劣质氮源，如硝酸钠，在缺乏细胞壁蛋白AoMad1在A。寡孢菌。为了表征硝酸盐在陷阱形成中的作用，我们敲除了硝酸盐同化途径中的四个基因，并评估了硝酸盐、宿主线虫或两者对突变体和野生型陷阱形成的可能影响。结果表明，硝酸盐诱导的陷阱使用一个独特的途径，不同的诱导线虫。此外，在线虫存在下，硝酸盐促进陷阱形成。我们的研究结果表明，线虫或硝酸盐诱导的陷阱的形成是受硝酸盐同化途径。(C)2016由Elsevier Inc.出版
Nutrient conditions are important for trap formation and a switch from saprophytic to predacious lifestyle in nematode-trapping fungi. Arthrobotrys oligospora is a typical nematode-trapping fungus which can capture and kill nematodes by means of traps formed in response to the presence of their prey. A nutrient-limited environment was previously shown to be important for trap formation, which was induced by several inferior nitrogen sources, such as sodium nitrate, in the absence of the cell wall protein AoMad1 in A. oligospora. To characterize the role of nitrate in trap formation, we knocked out four genes in the nitrate assimilation pathway and assessed possible effect of nitrate, host nematode or both on the trap formation in the mutants and the wild type. The results showed that trap induction by nitrate used a distinct pathway that differed from induction by nematodes. Moreover, the nitrate facilitated trap formation in the presence of nematodes. Our findings revealed that the trap formation induced by nematodes or nitrate is influenced by the nitrate assimilation pathway. (C) 2016 Published by Elsevier Inc.