Gene regulation of melatonin and dopamine receptors during eye development
Gene regulation of melatonin and dopamine receptors during eye development
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DOI：
10.1016/s0306-4522(03)00298-7
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Brown, GM
中科院分区：
文献类型：
--
作者：
Fujieda, H;Scher, J;Brown, GM
为了深入了解褪黑激素和多巴胺在视网膜发育中的作用，通过逆转录-聚合酶链反应分析了不同发育阶段大鼠眼中两种褪黑激素受体MT 1和MT 2以及五种多巴胺受体D-1、D-2、D-3、D-4和D-5的基因表达。MT 1转录水平在胚胎第16天（E）达到最高水平，然后逐渐下降，直到出生后第14天（P）达到成人水平。MT 2转录水平在E16时达到峰值，但随后急剧下降，直到出生时降至最低水平，并在整个出生后时期保持不变。因此，MT 1和MT 2受体的基因表达与眼睛的成熟呈显著的负相关。与褪黑激素受体相反，所有多巴胺受体亚型的基因表达，除了D-3，只显示出增加的发展过程中，在成年期的最高水平。在整个发育期间没有检测到D-3的消息。D-1样受体D-1和D-5的基因表达分别在E16和E20的胎儿期显著增加至成人水平。D-2样受体，D-2和D-4，另一方面，转录水平在出生前没有检测到，但增加到成人水平的P7和P14，分别显着目前的研究结果表明，存在独特的发展机制，各种G蛋白偶联受体的转录调节的眼睛。(C)2003年IBRO。由Elsevier Science Ltd.出版，版权所有。
To gain insight into the role of melatonin and dopamine in retinal development, gene expression of two melatonin receptors, MT1, and MT2, as well as five dopamine receptors, D-1, D-2, D-3, D-4 and D-5, in the rat eye was analyzed by reverse transcription-polymerase chain reaction across various developmental stages. MT1, transcript levels reached maximum levels at embryonic day (E) 16 and then decreased gradually until reaching adult levels by postnatal day (P) 14. MT2 transcript levels similarly peaked at E16, but then decreased dramatically until birth to its lowest levels, which were maintained throughout the postnatal period. Thus, gene expression of both the MT1 and MT2 receptors showed a striking inverse correlation with maturation of the eye. In contrast to melatonin receptors, gene expression of all dopamine receptor subtypes, except for D-3, showed only an increase as development proceeds with highest levels in adulthood. The D-3 message was not detected throughout the developmental period examined. Gene expression of D-1-like receptors, D-1 and D-5, showed a substantial increase to adult levels during the fetal period at E16 and E20, respectively. Transcript levels of D-2-like receptors, D-2 and D-4, on the other hand, were not detected before birth but increased significantly to adult levels by P7 and P14, respectively.The present findings suggest the presence of unique developmental mechanisms by which transcription of various G protein-coupled receptors are regulated in the eye. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.