CHARACTERIZATION OF PERFLUOROSULFONIC ACID POLYMER COATED ENZYME ELECTRODES AND A MINIATURIZED INTEGRATED POTENTIOSTAT FOR GLUCOSE ANALYSIS IN WHOLE-BLOOD
CHARACTERIZATION OF PERFLUOROSULFONIC ACID POLYMER COATED ENZYME ELECTRODES AND A MINIATURIZED INTEGRATED POTENTIOSTAT FOR GLUCOSE ANALYSIS IN WHOLE-BLOOD
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DOI：
10.1021/ac00170a003
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发表时间：
1988-10-01
影响因子：
7.4
通讯作者：
BALTES, HP
中科院分区：
文献类型：
--
作者：
HARRISON, DJ;TURNER, RFB;BALTES, HP
The perfluorosulfonic acid polymer Nafion leads to highly reproducible electrode preparation and performance when used as a dialysis membrane material on glucose oxidase/platinum electrodes in the deetermination of glucose in whole blood. Protection from enzyme electrode fouling in whole blood was demonstrated for a 1.7 .mu.m thick perfluorosulfonic acid polymer overcoat by 6 days of continuous in vitro measurement in fresh whole blood samples at 37.degree. C, during which time the glucose calibration slope decreased by .apprx. 6%. Coating with the perfluorosulfonic acid polymer yields enzyme electrodes with a linear response to glucose up to at least 28 mM and response times of 5-17 s, superior to those for electrodes coated with cellulose dialysis membranes. A custom designed, CMOS integrated, two-electrode potentiostat that also measures electrode current and provides an output of 75 mV/.mu.A is presented as a prototype for a miniaturized glucose sensor. The lower limit of detection is < 2 nA, and linear response over the range of 10 nA to 2 .mu.A is observed for the electronic device. The differential mode capability of the circuit permits elimination of background current due to interfering species in whole blood. The accuracy of the electrode-sensor combination is .+-. 2% relative to a Beckman glucose analyzer.
