The effect of legume supplementation on the gut microbiota in rural Malawian infants aged 6 to 12 months.
The effect of legume supplementation on the gut microbiota in rural Malawian infants aged 6 to 12 months.
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DOI：
10.1093/ajcn/nqaa011
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发表时间：
2020-02
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通讯作者：
M. I. Ordiz;Stefan Janssen;G. Humphrey;G. Ackermann;Kevin B. Stephenson;Sophia E. Agapova;O. Divala;Yankho Kaimila;K. Maleta;Caroline Zhong;R. Knight;Indi Trehan;P. Tarr;B. Rusconi;M. Manary
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作者：
M. I. Ordiz;Stefan Janssen;G. Humphrey;G. Ackermann;Kevin B. Stephenson;Sophia E. Agapova;O. Divala;Yankho Kaimila;K. Maleta;Caroline Zhong;R. Knight;Indi Trehan;P. Tarr;B. Rusconi;M. Manary
菜豆和豇豆含有约25%的蛋白质和25%的纤维，在撒哈拉以南非洲地区被推荐为补充食品。目的：本研究的目的是确定马拉维6至12个月婴儿每日补充豆类是否会改变粪便微生物群的16 S构型，如扩增子序列变体（ASV）所示。本研究是在随机、双盲、对照临床试验的背景下进行的，以评估豇豆或普通豆类补充剂是否降低肠道通透性或增加线性生长。有2个村庄进行了研究。在≤6个时间点对236名婴儿的新鲜粪便进行快速冷冻。使用地球微生物组项目方案对粪便进行测序，并使用Qiime和Qiita，开源，验证的软件包处理数据。α-多样性是使用信仰的测试来测量的。通过测定ASV的加权UniFrac距离并使用置换多变量ANOVA进行比较来表征16 S构型。结果：在分析的1249份样本中，开始补充豆类后，受试者粪便微生物组的α多样性没有变化。无论是豇豆，也没有改变普通豆类的整体16 S配置在任何年龄。16 S构型在6 ~ 9个月线性生长良好和线性生长不良的儿童之间存在差异，但没有特异性ASV相对丰度差异。在9个月时，肠道通透性正常和异常的儿童之间的16 S构型不同，但没有特异性ASV的相对丰度不同。在与不同16 S构型相关的种群分类特征中，村庄聚集最为明显。结论：在母乳喂养的非洲农村婴儿中，豆类补充剂直到9个月大才影响肠道微生物群落的结构。该试验在clinicaltrials.gov上注册为NCT 02472262。
BACKGROUND Common bean and cowpea contain about 25% protein and 25% fiber, and are recommended as complementary foods in sub-Saharan Africa. OBJECTIVE The objective of this study was to determine if a daily legume supplement given to Malawian infants aged 6 to 12 mo alters the 16S configuration of the fecal microbiota as read out by amplicon sequence variants (ASVs). METHODS This study was conducted within the context of a randomized, double-blind, controlled clinical trial to assess whether cowpea or common bean supplementation reduced intestinal permeability or increased linear growth. There were 2 village clusters in which the study was conducted. Fresh stool collections were flash frozen from 236 infants at ≤6 time points. The stools were sequenced using Earth Microbiome project protocols and data were processed using Qiime and Qiita, open-source, validated software packages. α-diversity was measured using the Faith's test. The 16S configuration was characterized by determining the weighted UniFrac distances of the ASVs and comparing them using permutational multivariate ANOVA. RESULTS Among the 1249 samples analyzed, the α-diversity of the fecal microbiome was unchanged among subjects after initiation of legume supplementation. Neither cowpea nor common bean altered the overall 16S configuration at any age. The 16S configuration differed between children with adequate and poor linear growth aged from 6 to 9 mo, but no specific ASVs differed in relative abundance. The 16S configuration differed between children with normal and abnormal intestinal permeability at 9 mo, but no specific ASVs differed in relative abundance. Among categorical characteristics of the population associated with different 16S configurations, village cluster was most pronounced. CONCLUSION Legume supplementation in breastfed, rural African infants did not affect the structure of the gut microbial communities until the children were aged 9 mo. This trial was registered at clinicaltrials.gov as NCT02472262.