Characterization of the Aspergillus nidulans Monodictyphenone Gene Cluster
Characterization of the Aspergillus nidulans Monodictyphenone Gene Cluster
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DOI：
10.1128/aem.02187-09
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发表时间：
2010-04-01
影响因子：
4.4
通讯作者：
Oakley, Berl R.
中科院分区：
文献类型：
--
作者：
Chiang, Yi-Ming;Szewczyk, Edyta;Oakley, Berl R.
缺失cclA，构巢曲霉的COMPASS复合物的组分，导致产生单网酚和大黄素衍生物。通过在cclA缺失菌株中的一组靶向缺失，我们已经确定了单网酚酮和大黄素类似物生物合成所需的基因。从mdpH Delta菌株中鉴定出一种中间体内藏红花素，表明mdpH可能编码一种脱羧酶。此外，通过用可诱导的alcA启动子替换mdpA（推定的aflJ同源物）和mdpE（推定的aflR同源物）的启动子，我们已经证实了MdpA作为共激活因子起作用。基于生物信息学分析和生物合成中间体的表征，我们提出了一条单大黄酚和大黄素衍生物的生物合成途径。
Deletion of cclA, a component of the COMPASS complex of Aspergillus nidulans, results in the production of monodictyphenone and emodin derivatives. Through a set of targeted deletions in a cclA deletion strain, we have identified the genes required for monodictyphenone and emodin analog biosynthesis. Identification of an intermediate, endocrocin, from an mdpH Delta strain suggests that mdpH might encode a decarboxylase. Furthermore, by replacing the promoter of mdpA ( a putative aflJ homolog) and mdpE ( a putative aflR homolog) with the inducible alcA promoter, we have confirmed that MdpA functions as a coactivator. We propose a biosynthetic pathway for monodictyphenone and emodin derivatives based on bioinformatic analysis and characterization of biosynthetic intermediates.