Elucidating response mechanisms at the metabolic scale of Eisenia fetida in typical oil pollution sites: A native driver in influencing carbon flow
Elucidating response mechanisms at the metabolic scale of Eisenia fetida in typical oil pollution sites: A native driver in influencing carbon flow
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DOI：
10.1016/j.envpol.2023.122545
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发表时间：
2023-09-20
影响因子：
8.9
通讯作者：
Zhou，Qixing
中科院分区：
文献类型：
--
作者：
Hou，Zelin;Mo，Fan;Zhou，Qixing
先前对实际石油碳氢化合物（PH）污染系统中蚯蚓（Eisenia fetida）应激反应模式的研究较少关注。因此，本研究基于代谢组学和组织学观察，探讨PH污染对蚯蚓的生态毒理学影响，并进行蚯蚓代谢、PH类型和浓度、土壤理化特征以及微生物群落结构（即多样性和丰度）和功能之间的相关性分析。结果表明，由于PH有机物丰富，PH污染条件下蚯蚓的细胞代谢发生变化，导致它们使用有机酸作为替代能源（即糖异生途径）。同时，还鉴定了与细胞摄取、应激反应和膜紊乱相关的生物标志物代谢物。此外，与对照组相比，观察到相当大的表皮层和角质层破坏以及 PH 内化。结果表明，PH 污染优先通过间接（即微生物代谢调节）而非直接（即与蚯蚓直接相互作用）机制影响蚯蚓的生理稳态。此外，验证了不同的二氧化碳释放量，这凸显了蚯蚓在影响碳转化方面的潜在作用，并与相当丰富的能量代谢相关途径相对应。本研究表明，PH值污染可以通过直接和间接机制引起蚯蚓强烈的应激反应，进而显着影响PH值污染地点的碳转化。
Previous investigations on the stress response patterns of earthworms (Eisenia fetida) in practical petroleum hydrocarbon (PH) contamination systems were less focused. Therefore, this study investigated the ecotoxicological effect of PH contamination on earthworms based on metabonomics and histological observation, followed by correlation analysis between the earthworm metabolism, PH types and concentrations, soil physicochemical characteristics, and the microbial community structures (i.e., diversity and abundance) and functions. The results showed that due to the abundant PH organics, the cell metabolism of earthworms shifts under PH contamination conditions, leading them to use organic acids as alternative energy sources (i.e., gluconeogenesis pathway). Simultaneously, biomarker metabolites related to cellular uptake, stress response, and membrane disturbance were identified. In addition, when compared to the controls, considerable epicuticle and cuticle layer disruption was observed, along with PH internalization. It was demonstrated that PH pollution preferentially influences the physiological homeostasis of earthworms through indirect (i.e., microbial metabolism regulation) than direct (i.e., direct interaction with earthworms) mechanisms. Moreover, the varied CO2releasement was verified, which highlights the potential role of earthworms in influencing carbon transformation and corresponds with the considerably enriched energy metabolism-related pathway. This study indicated that PH contamination can induce a strong stress response in earthworms through both direct and indirect mechanisms, which in turn, substantially influences carbon transformation in PH contamination sites.