Differential transcriptomic responses of ancient and modern Daphnia genotypes to phosphorus supply
Differential transcriptomic responses of ancient and modern Daphnia genotypes to phosphorus supply
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DOI：
10.1111/mec.13009
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发表时间：
2015-01
影响因子：
4.9
通讯作者：
Priyanka Roy Chowdhury;Dagmar Frisch;D. Becker;Jacqueline Lopez;L. Weider;J. Colbourne;P. Jeyasingh
中科院分区：
文献类型：
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作者：
Priyanka Roy Chowdhury;Dagmar Frisch;D. Becker;Jacqueline Lopez;L. Weider;J. Colbourne;P. Jeyasingh
很少有人知道的作用，转录组的变化，在驱动自然种群的表型进化，特别是在应对人为环境变化。先前使用复活生态学方法对湖泊中几个世纪的进化分开的水蚤基因型进行的分析揭示了惊人的遗传和表型变化，这些变化与人为环境变化高度相关，特别是磷（P）驱动的营养物富集（即富营养化）。在这里，我们使用微阵列比较了两个古代（~700岁）和两个现代（~10岁）基因型在历史（低P）和当代（高P）环境条件下的转录组。我们发现相当大的转录组之间的变化“古代”和“现代”的基因型在这两种治疗方法，与压力（低P）条件下引发差异表达（DE）的大量基因。此外，更多的基因DE之间的“古代”和“现代”的基因型比在这些群体。不同基因型的个体基因表达模式差异很大，这表明不同的转录组反应可能导致相似的表型。虽然这在基因水平上混淆了“古代”和“现代”基因型之间的模式，但在功能水平上模式是可辨别的：DE基因的注释揭示了参与代谢途径的基因在响应P处理时的特别富集。对基因家族的分析表明，已知在水蚤和其他生物中处理P限制的重要途径中存在显著的DE。这种观察基因型的一个单一的自然人口，分开数百年的进化在对比的环境条件之前和期间的人为环境变化，突出了重要的作用，转录机制的进化反应的人口。
Little is known about the role of transcriptomic changes in driving phenotypic evolution in natural populations, particularly in response to anthropogenic environmental change. Previous analyses of Daphnia genotypes separated by centuries of evolution in a lake using methods in resurrection ecology revealed striking genetic and phenotypic shifts that were highly correlated with anthropogenic environmental change, specifically phosphorus (P)‐driven nutrient enrichment (i.e. eutrophication). Here, we compared the transcriptomes of two ancient (~700‐year‐old) and two modern (~10‐year‐old) genotypes in historic (low P) and contemporary (high P) environmental conditions using microarrays. We found considerable transcriptomic variation between ‘ancient’ and ‘modern’ genotypes in both treatments, with stressful (low P) conditions eliciting differential expression (DE) of a larger number of genes. Further, more genes were DE between ‘ancient’ and ‘modern’ genotypes than within these groups. Expression patterns of individual genes differed greatly among genotypes, suggesting that different transcriptomic responses can result in similar phenotypes. While this confounded patterns between ‘ancient’ and ‘modern’ genotypes at the gene level, patterns were discernible at the functional level: annotation of DE genes revealed particular enrichment of genes involved in metabolic pathways in response to P‐treatments. Analyses of gene families suggested significant DE in pathways already known to be important in dealing with P‐limitation in Daphnia as well as in other organisms. Such observations on genotypes of a single natural population, separated by hundreds of years of evolution in contrasting environmental conditions before and during anthropogenic environmental changes, highlight the important role of transcriptional mechanisms in the evolutionary responses of populations.