Susceptibility of laboratory rats against genotypes 1, 3, 4, and rat hepatitis E viruses
Susceptibility of laboratory rats against genotypes 1, 3, 4, and rat hepatitis E viruses
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DOI：
10.1016/j.vetmic.2012.12.014
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发表时间：
2013-04-12
影响因子：
3.3
通讯作者：
Wakita, Takaji
中科院分区：
文献类型：
--
作者：
Li, Tian-Cheng;Yoshizaki, Sayaka;Wakita, Takaji
为了确定大鼠是否对戊型肝炎病毒（HEV）易感，每组3只实验大鼠（Wistar）通过静脉注射分别接种基因型1、3、4和大鼠HEV。采集血清和粪便标本，分别采用RT-PCR和ELISA检测HEV RNA和抗HEV抗体。接种后3个月监测病毒感染情况。从接种G1、G3或G4型HEV的大鼠收集的血清或粪便样本均未显示病毒复制阳性迹象。虽然ALT水平未见变化，但接种大鼠HEV的两只大鼠的粪便中均检测到大鼠HEV RNA，接种后3周，两只大鼠的抗大鼠HEV IgG和IgM均呈阳性。这些结果表明，大鼠对大鼠HEV易感，但对GI、G3和G4型HEV不易感。我们还证实了裸鼠对获得大量大鼠HEV是有用的，并且大鼠HEV是通过粪口途径传播的。(C) 2012 Elsevier B.V.版权所有
To determine whether or not rats are susceptible to hepatitis E virus (HEV) infection, each of group containing three laboratory rats (Wistar) were experimentally inoculated with genotypes 1, 3, 4 and rat HEV by intravenous injection. Serum and stool samples were collected and used to detect HEV RNA and anti-HEV antibodies by RT-PCR and ELISA, respectively. The virus infection was monitored up to 3 months after inoculation. None of the serum or stool samples collected from the rats inoculated with G1, G3, or G4 HEV indicated positive sign for virus replication. Although no alteration was observed in ALT level, rat HEV RNA was detected in stools from both of the rats inoculated with rat HEV, and both rats were positive for anti-rat HEV IgG and IgM from 3 weeks after inoculation. These results demonstrated that rats are susceptible to rat HEV but not to GI, G3, and G4 HEV. We also confirm that the nude rats were useful for obtaining a large amount of rat HEV and that the rat HEV was transmitted by the fecal-oral route. (C) 2012 Elsevier B.V. All rights reserved.