The Effect of Gold Nanoparticles on the Proliferation and Differentiation of Murine Osteoblast: A Study of MC3T3-E1 Cells In Vitro
The Effect of Gold Nanoparticles on the Proliferation and Differentiation of Murine Osteoblast: A Study of MC3T3-E1 Cells In Vitro
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纳米金对小鼠成骨细胞增殖和分化的影响：MC3T3-E1细胞的体外研究
DOI：
10.1166/jnn.2014.8717
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发表时间：
2014-07-01
影响因子：
--
通讯作者：
Chen, Fengshan
中科院分区：
文献类型：
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作者：
Yao, Yuanyuan;Shi, Xiujuan;Chen, Fengshan
目前的研究涉及金纳米颗粒（AuNP）最佳尺寸和浓度的识别和分子水平表征。使用透射电子显微镜 (TEM) 和动态光散射 (DLS) 合成并表征了稳定的金纳米颗粒。采用 MTT 细胞增殖测定评估金纳米颗粒对前成骨细胞 MC3T3-E1 增殖的浓度和尺寸依赖性影响。结果表明，浓度为10(-11) ppm的直径30 nm的金纳米粒子对促进细胞增殖最有效。还使用碱性磷酸酶（ALP）活性测定和ALP染色来确认金纳米颗粒对成骨细胞增殖和分化的影响。此外，使用逆转录聚合酶链反应（RT-PCR）测量成骨基因Runx2、ALP、OCN和OPN的表达作为响应金纳米颗粒。数据表明，浓度为10(-11) ppm的30 nm金纳米粒子是促进成骨细胞增殖和分化的最佳尺寸和浓度组合，如ALP活性和成骨基因Runx2、ALP、OCN和OPN的表达增加所表明的。总的来说，这项研究的结果表明，金纳米粒子可以促进成骨细胞的增殖和分化，并可有效用于促进骨再生的治疗。
The current study involves in identification and molecular levels characterization of optimal size and concentration of gold nanoparticles (AuNPs). Stable, gold nanoparticles were synthesized and characterized using transmission electron microscopy (TEM) and dynamic light scattering (DLS). The concentration and size dependent effects of the gold nanoparticles on proliferation of pre-osteoblast cells MC3T3-E1 was evaluated employing MTT cell proliferation assay. The results revealed that 30 nm diameter gold nanoparticles at a concentration of 10(-11) ppm were the most effective in promoting cell proliferation. Assay for alkaline phosphatase (ALP) activity and ALP staining were also used to confirm the effect of gold nanoparticles on osteoblast proliferation and differentiation. Moreover, reverse transcriptase polymerase chain reaction (RT-PCR) was used to measure the expression of the osteogenic genes Runx2, ALP, OCN and OPN as response gold nanoparticles. The data demonstrated that 30 nm gold nanoparticles at a concentration of 10(-11) ppm was the best combination of size and concentration to promote the proliferation and differentiation of osteoblasts, as indicated by an increase in the ALP activity and expression of the osteogenic genes Runx2, ALP, OCN and OPN. Collectively the results of this study suggest that gold nanoparticles can promote the proliferation and differentiation of osteoblasts and could be used effectively in treatments promoting bone regeneration.