Biphasic responses of human vascular smooth muscle cells to magnesium ion.
Biphasic responses of human vascular smooth muscle cells to magnesium ion.
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DOI：
10.1002/jbm.a.35570
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Zhu D
中科院分区：
文献类型：
--
作者：
Ma J;Zhao N;Zhu D
镁基合金在可生物降解心血管支架中具有广阔的应用前景。镁合金支架的降解产物可能对周围血管细胞产生显著影响。然而，镁离子与血管细胞之间的相互作用在分子和细胞水平上的知识仍然很大程度上缺失。血管平滑肌细胞（SMC）在支架植入后再狭窄和伤口愈合的发病机制中起重要作用。本研究探讨了细胞外镁离子（Mg 2+）对平滑肌细胞行为的短期影响。细胞对Mg 2+的反应呈浓度依赖性的双相性。低浓度（10 mM）的Mg 2+增加细胞活力，细胞增殖率，细胞粘附，细胞铺展，细胞迁移率和肌动蛋白表达。相比之下，较高浓度（40-60 mM）的Mg 2+对细胞有有害影响。基因表达分析显示，Mg 2+改变了主要与细胞粘附、细胞损伤、血管生成、炎症、凝血和细胞生长相关的基因的表达。这项研究的发现提供了一些有价值的信息SMC对镁离子在细胞和分子水平的反应，并为未来的支架材料的镁控制释放的指导。
Magnesium-based alloys are promising in biodegradable cardiovascular stent applications. The degradation products of magnesium stents may have significant impacts on the surrounding vascular cells. However, knowledge on the interactions between magnesium ion and vascular cells at the molecular and cellular levels is still largely missing. Vascular smooth muscle cell (SMC) plays an important role in the pathogenesis of restenosis and wound healing after stent implantation. This study evaluated the short-term effects of extracellular magnesium ion (Mg2+) on the cellular behaviors of SMCs. Cellular responses to Mg2+ were biphasic and in a concentration-dependent manner. Low concentrations (10 mM) of Mg2+ increased cell viability, cell proliferation rate, cell adhesion, cell spreading, cell migration rate, and actin expression. In contrast, higher concentrations (40–60 mM) of Mg2+ had deleterious effects on cells. Gene expression analysis revealed that Mg2+ altered the expressions of genes mostly related to cell adhesion, cell injury, angiogenesis, inflammation, coagulation, and cell growth. Finding from this study provides some valuable information on SMC responses toward magnesium ions at the cellular and molecular levels, and guidance for future controlled release of magnesium from the stent material.