Tricarboxylic acid cycle of glia in the in vivo human brain
Tricarboxylic acid cycle of glia in the in vivo human brain
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DOI：
10.1002/nbm.725
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发表时间：
2002-02-01
影响因子：
2.9
通讯作者：
Ross, BD
中科院分区：
文献类型：
--
作者：
Blüml, S;Moreno-Torres, A;Ross, BD
在大脑中，醋酸盐只被神经胶质氧化。为了确定神经胶质代谢对三羧酸循环（TCA）的贡献，在6项研究中，在3名正常成人受试者和1名接受丙戊酸控制癫痫发作的癫痫患者中输注了1-C-13-乙酸酯。在60分钟内静脉输注吨克99%1-C-13标记的乙酸盐作为3.3%w/v溶液，在此期间获得脑的体内C-13 MR谱。正如预期的那样，13 C标记快速富集脑碳酸氢盐、谷氨酸盐和谷氨酰胺C-5。根据禁食正常受试者碳酸氢盐的稳态C-13富集计算的乙酸盐氧化的平均速率为0.13 +/- 0.03 mumol/g/min（n = 4），约为总脑TCA循环速率的20%。版权所有（C）2002约翰威利父子有限公司
In the brain, acetate is exclusively oxidized by glia. To determine the contribution of glial metabolism to the tricarboxylic acid cycle (TCA), 1-C-13-acetate was infused in six studies in three normal adult subjects and one epileptic receiving valproic acid for seizure control. Ton grams of 99% 1-C-13 labeled acetate were infused intravenously as a 3.3% w/v solution over 60 min, during which in vivo C-13 MR spectra of the brain were acquired. As expected, 13C label rapidly enriched cerebral bicarbonate, glutamate and glutamine C-5. The mean rate of acetate oxidation calculated from steady-state C-13 enrichment of bicarbonate in fasted normal subjects was 0.13 +/- 0.03 mumol/g/min (n = 4), approximately 20% of the total cerebral TCA cycle rate. Copyright (C) 2002 John Wiley Sons, Ltd.