Inhibitory effect of hexane fraction from Myagropsis myagroides on pancreatic α-amylase in vitro.
Inhibitory effect of hexane fraction from Myagropsis myagroides on pancreatic α-amylase in vitro.
复制标题
来自 Myagropsis myagroides 的己烷组分对体外胰腺 α-淀粉酶的抑制作用。
DOI：
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发表时间：
2015
影响因子：
2.8
通讯作者：
D. Ahn
中科院分区：
文献类型：
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作者：
W. Pak;Koth;Min;Ji;D. Ahn
Myagropsis myagroides（Mm）甲醇提取物在5 mg/ml的浓度下显示出13%的α-淀粉酶抑制活性。结果表明，Mm甲醇提取物正己烷组分对α-淀粉酶具有抑制活性，IC 50值为4.24 mg/ml。正己烷馏分用硅胶柱层析分离，得到6个亚馏分。结果表明，CHCl_3：MeOH = 50：1的洗脱液对α-淀粉酶的抑制活性最高，IC_（50）为0.72 mg/ml。该馏分用Sephadex LH-20柱层析和十八烷基硅胶（ODS）Sepak柱纯化，得到7个亚馏分。分数（Fr.）4对α-淀粉酶也有较强的抑制作用，IC_（50）为0.75 mg/ml。Fr.4经Sephadex LH-20柱层析和ODS Sepak柱层析得到6个亚组分。Fr. 4-2被鉴定为Sargachromanol I，IC 50值为0.40 mg/ml，从Lineweaver-Burk图分析的抑制模式显示其为非竞争性抑制剂。这些结果表明，Mm具有作为天然抗糖尿病剂的潜力。
A Myagropsis myagroides (Mm) methanol extract showed α-amylase inhibitory activity of 13% at a concentration of 5 mg/ml. Results showed that the hexane fraction from the Mm methanol extract exhibited α-amylase inhibitory activity with an IC50 value of 4.24 mg/ml. The hexane fraction was separated using silica-gel column chromatography, and six subfractions were obtained. The fraction eluted with CHCl3:MeOH = 50:1 showed the highest α-amylase inhibitory activity with an IC50 value of 0.72 mg/ml. This fraction was purified using Sephadex LH-20 column chromatography and an octadecyl silica (ODS) Sepak cartridge, obtaining seven subfractions. Fraction (Fr.) 4 also showed a strong α-amylase inhibitory activity with an IC50 value of 0.75 mg/ml. Fr. 4 was purified by Sephadex LH-20 column chromatography and ODS Sepak cartridge, obtaining six subfractions. Fr. 4-2 was identified as sargachromanol I with an IC50 value of 0.40 mg/ml, and the inhibition pattern analyzed from Lineweaver-Burk plots revealed it to be an uncompetitive inhibitor. These results suggest that Mm has potential as a natural antidiabetes agent.