Immunogenicity Analysis of a Novel Subunit Vaccine Candidate Molecule-Recombinant L7/L12 Ribosomal Protein of Brucella suis
Immunogenicity Analysis of a Novel Subunit Vaccine Candidate Molecule-Recombinant L7/L12 Ribosomal Protein of Brucella suis
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DOI：
10.1007/s12010-016-2076-x
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发表时间：
2016-08-01
影响因子：
3
通讯作者：
Wang, Jian-Ying
中科院分区：
文献类型：
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作者：
Du, Zhi-Qiang;Li, Xin;Wang, Jian-Ying
布鲁氏菌是一种细胞内寄生虫，可感染特殊的家畜和人。家畜感染布鲁氏菌后，生殖系统会受到影响和破坏，造成巨大的经济损失。更严重的是，它可能会从牲畜传染给人类。到目前为止，还没有对人类足够安全的疫苗。在这一点上，亚单位疫苗成为攻克布鲁氏菌病的新突破口。本研究以布鲁氏菌rL 7/L12-BLS融合蛋白为抗原，免疫家兔，检测其免疫原性。兔抗血清抗体水平检测结果表明，rL 7/L12-BLS融合蛋白能诱导兔产生高水平的IgG。rL 7/L12组和rL 7/L12-BLS组兔抗血清中γ干扰素（IFN-γ）浓度均明显升高。实时荧光定量PCR（qRT-PCR）结果显示rL 7/L12组和rL 7/L12-BLS组IFN-γ基因表达水平均明显上调。这些结果表明，布鲁氏菌L7/L12蛋白是一种理想的亚单位疫苗候选物，具有良好的免疫原性。布氏杆菌光胺合酶（BLS）分子是抗原蛋白的良好转运载体。
Brucella was an intracellular parasite, which could infect special livestock and humans. After infected by Brucella, livestock's reproductive system could be affected and destroyed resulting in huge economic losses. More seriously, it could be contagious from livestock to humans. So far, there is no available vaccine which is safe enough for humans. On this point, subunit vaccine has become the new breakthrough of conquering brucellosis. In this study, Brucella rL7/L12-BLS fusion protein was used as an antigen to immunize rabbits to detect the immunogenicity. The results of antibody level testing assay of rabbit antiserum indicated rL7/L12-BLS fusion protein could elicit rabbits to produce high-level IgG. And gamma interferon (IFN-gamma) concentrations in rabbit antiserum were obviously up-regulated in both the rL7/L12 group and rL7/L12-BLS group. Besides, the results of quantitative real-time PCR (qRT-PCR) showed the IFN-gamma gene's expression levels of both the rL7/L12 group and rL7/L12-BLS group were obviously up-regulated. All these results suggested Brucella L7/L12 protein was an ideal subunit vaccine candidate and possessed good immunogenicity. And Brucella lumazine synthase (BLS) molecule was a favorable transport vector for antigenic protein.