Bacteroides forsythus hemagglutinin is inhibited by N-acetylneuraminyllactose
Bacteroides forsythus hemagglutinin is inhibited by N-acetylneuraminyllactose
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DOI：
10.1046/j.0902-0055.2001.00093.x
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发表时间：
2002-04-01
影响因子：
--
通讯作者：
Oppenheim, FG
中科院分区：
文献类型：
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作者：
Murakami, Y;Higuchi, N;Oppenheim, FG
福赛斯类杆菌是一种与牙周炎相关的病原体，它能产生一种血凝素。血凝素定位于福赛斯乳杆菌的包膜内。低至1 mM的乳糖和100 mM的L-精氨酸、L-赖氨酸均能抑制其血凝活性。N-乙酰神经氨基乳糖(NeuAc-乳糖)的抑制作用至少是乳糖的100倍，因为它在低于10微米的浓度下完全抑制血凝。这类似于幽门螺杆菌血凝素。这种血凝素不耐热，不能用胰酶等酶处理。针对主要的物种特异性蛋白之一的S层蛋白产生的特异性抗体对血凝集没有抑制作用。这些结果表明，连翘对NeuAc-乳糖敏感的粘附素可能在口腔定植中起重要作用。
Bacteroides forsythus, which has been recognized as a pathogen associated with periodontitis, produces a hemagglutinin. The hemagglutinin was localized in the envelope of B. forsythus. The hemagglutinating activity was inhibited by lactose at concentrations as low as 1 mM, and by L-arginine and L-lysine at concentrations of 100 mM. N-Acetylneuraminyllactose (NeuAc-lactose) was at least 100 times more potent an inhibitor than lactose, as it completely inhibited the hemagglutination at concentrations below 10 muM. This is similar to the Helicobacter pylorl hemagglutinin. The hemagglutinin was heat-labile, and resistant to treatment with proteases such as trypsin. A specific antibody raised against one of the S-layer proteins that are major species-specific proteins had no inhibitory effect on the hemmaglutination. These results suggest that the NeuAc-lactose-sensitive adhesin of B. forsythus may play an important role in colonization in the oral cavity.