Mass cytometry staining for human bone marrow clinical samples.
Mass cytometry staining for human bone marrow clinical samples.
复制标题
DOI：
10.1016/j.xpro.2022.101163
复制
发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Baginska J
中科院分区：
文献类型：
--
作者：
Hallisey M;Dennis J;Abrecht C;Pistofidis RS;Schork AN;Lightbody ED;Heilpern-Mallory D;Severgnini M;Ghobrial IM;Hodi FS;Baginska J
该方案详细描述了使用质谱细胞计数优化用于人骨髓免疫群体免疫表型分析的染色技术。该方案考虑了使用人骨髓工作的局限性，例如活力降低、细胞计数低和细胞团易碎，以成功获得准备用于下游分析的单个活细胞。该测定可用于表征免疫群体的活化、耗竭和细胞毒性，并确保对人骨髓临床样本进行全面的免疫表型分析。用于评估人骨髓临床样本中免疫细胞的染色方案用于区分主要免疫群体的经验证的质谱细胞计数组对多参数质谱细胞计数数据分析的设门策略的见解该方案详细介绍了一种染色技术，该技术使用质谱细胞计数优化了人骨髓免疫群体的免疫表型。该方案考虑了使用人骨髓工作的局限性，例如活力降低、细胞计数低和细胞团易碎，以成功获得准备用于下游分析的单个活细胞。该测定可用于表征免疫群体的活化、耗竭和细胞毒性，并确保对人骨髓临床样本进行全面的免疫表型分析。
This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully acquire single viable cells ready for downstream analysis. This assay can be used to characterize the activation, exhaustion, and cytotoxicity of immune populations and ensure comprehensive immunophenotyping of human bone marrow clinical samples. Staining protocol for assessment of immune cells in human bone marrow clinical samples Validated mass cytometry panel for distinction of major immune populations Insights on gating strategy for multiparameter mass cytometry data analysis This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully acquire single viable cells ready for downstream analysis. This assay can be used to characterize the activation, exhaustion, and cytotoxicity of immune populations and ensure comprehensive immunophenotyping of human bone marrow clinical samples.