A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule
A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule
复制标题
使用磷酸盐捕获分子对血液和固体组织中的鞘氨醇和神经酰胺-1-磷酸进行质谱分析的净化方法
DOI：
10.1007/7651_2017_6
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发表时间：
2017
影响因子：
--
通讯作者：
Satouchi Kiyoshi
中科院分区：
文献类型：
--
作者：
Morishige Jun-ichi;Yamashita Ryouhei;Tanaka Tamotsu;Satouchi Kiyoshi
显示了使用磷酸捕获分子的鞘氨醇-1-磷酸（S1 P）的质谱技术和质谱测定。该方案是快速的，既不需要薄层色谱法，也不需要液相色谱法，并适用于血液和固体组织样品。质谱法也适用于神经酰胺-1-磷酸。
Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate (S1P) using a phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples. The mass spectrometric method is also applicable to ceramide-1-phosphate.