RNA segment 9 exists as a duplex concatemer in an Australian strain of epizootic haemorrhagic disease virus (EHDV): Genetic analysis and evidence for the presence of concatemers as a normal feature of orbivirus replication
RNA segment 9 exists as a duplex concatemer in an Australian strain of epizootic haemorrhagic disease virus (EHDV): Genetic analysis and evidence for the presence of concatemers as a normal feature of orbivirus replication
复制标题
DOI：
10.1016/j.virol.2011.09.009
复制
发表时间：
2011-11-25
期刊：
影响因子：
3.7
通讯作者：
Mertens, P. P. C.
中科院分区：
文献类型：
--
作者：
Anthony, S. J.;Darpel, K. E.;Mertens, P. P. C.
报道了一株血清5型猪流行性出血病病毒（EHDV）的串联RNA。测序结果表明，串联RNA包含两个相同的基因组片段9的全长拷贝，序列排列，显然取代了片段的单体形式。体外翻译表明，串联RNA可以作为VP6翻译的可行模板，但不会产生双倍大小的蛋白。研究还评估了突变是否容易被引入第二拷贝（这可能表明一个串联RNA片段的潜在进化意义），然而多次传代（n = 40）没有产生上游或下游片段序列的变化。此外，我们提出的结果表明，在不同的轨道病毒的多个片段中（在组织培养和纯化病毒中）存在串联体或部分基因复制，这表明它们的产生可能是轨道病毒复制的正常特征。(C) 2011爱思唯尔公司版权所有。
This paper reports a concatemeric RNA in a strain of epizootic haemorrhagic disease virus (EHDV) serotype 5. Sequencing showed that the concatemeric RNA contains two identical full-length copies of genome segment 9, arranged in series, which has apparently replaced the monomeric form of the segment. In vitro translation demonstrated that the concatemeric RNA can act as a viable template for VP6 translation, but that no double-sized protein is produced. Studies were also performed to assess whether mutations might be easily introduced into the second copy (which might indicate some potential evolutionary significance of a concatemeric RNA segment), however multiple (n = 40) passages generated no changes in the sequence of either the upstream or downstream segments. Further, we present results that demonstrate the presence of concatemers or partial gene duplications in multiple segments of different orbiviruses (in tissue culture and purified virus), suggesting their generation is likely to be a normal feature of orbivirus replication. (C) 2011 Elsevier Inc. All rights reserved.