Multinucleated rat alveolar macrophages express functional receptors for calcitonin.
Multinucleated rat alveolar macrophages express functional receptors for calcitonin.
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多核大鼠肺泡巨噬细胞表达降钙素功能性受体。
DOI：
10.1152/ajprenal.1991.261.6.f1026
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Rosenzweig,SA
中科院分区：
文献类型：
--
作者：
Vignery,A;Raymond,MJ;Qian,HY;Wang,F;Rosenzweig,SA
单核吞噬细胞的融合在体内自发发生，并分别导致慢性炎症部位或骨中的多核巨细胞或破骨细胞的分化。尽管破骨细胞负责骨吸收，但巨细胞在慢性炎症反应和肿瘤中的功能作用仍知之甚少。我们最近报道，多核巨噬细胞的质膜与破骨细胞的质膜一样，富含Na-K-腺苷三磷酸酶（ATP酶）。我们还观察到，它们的 Na-K-ATP 酶的定位仅限于体内和体外细胞质膜的非粘附域，从而对其组织施加功能极性。通过观察这一结果，我们希望研究这些细胞是否也像破骨细胞一样表达降钙素（CT）的功能性受体。为此，在体外融合肺泡巨噬细胞，分析其结构和功能与 CT 的关联，并与单核腹膜和肺泡巨噬细胞进行比较。有证据表明，多核肺泡巨噬细胞表达高拷贝数的 CT 功能受体。我们的结果还表明，肺泡巨噬细胞，很像腹膜巨噬细胞，表达降钙素基因相关肽的功能受体。多核大鼠肺泡巨噬细胞为研究 CT 受体提供了一种新的模型系统，降钙素可能控制巨细胞分化的局部免疫反应。
The fusion of mononuclear phagocytes occurs spontaneously in vivo and leads to the differentiation of either multinucleated giant cells or osteoclasts in chronic inflammatory sites or in bone, respectively. Although osteoclasts are responsible for resorbing bone, the functional role of giant cells in chronic inflammatory reactions and tumors remains poorly understood. We recently reported that the plasma membrane of multinucleated macrophages is, like that of osteoclasts, enriched in Na-K-adenosinetriphosphatases (ATPases). We also observed that the localization of their Na-K-ATPases is restricted to the nonadherent domain of the plasma membrane of cells both in vivo and in vitro, thus imposing a functional polarity on their organization. By following this observation, we wished to investigate whether these cells also expressed, like osteoclasts, functional receptors for calcitonin (CT). To this end, alveolar macrophages were fused in vitro, and both their structural and functional association with CT was analyzed and compared with those of mononucleated peritoneal and alveolar macrophages. Evidence is presented that multinucleated alveolar macrophages express a high copy number of functional receptors for CT. Our results also indicate that alveolar macrophages, much like peritoneal, express functional receptors for calcitonin gene-related peptide. It is suggested that multinucleated rat alveolar macrophages offer a novel model system to study CT receptors and that calcitonin may control local immune reactions where giant cells differentiate.