Membrane disruption and DNA binding of Staphylococcus aureus cell induced by a novel antimicrobial peptide produced by Lactobacillus paracasei subsp tolerans FX-6
Membrane disruption and DNA binding of Staphylococcus aureus cell induced by a novel antimicrobial peptide produced by Lactobacillus paracasei subsp tolerans FX-6
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DOI：
10.1016/j.foodcont.2015.06.044
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发表时间：
2016-01-01
期刊：
影响因子：
6
通讯作者：
Cao, Yong
中科院分区：
文献类型：
--
作者：
Miao, Jianyin;Zhou, Jianliang;Cao, Yong
在此之前，我们已经从藏灵菇中分离到一种新的细菌素--肽F1，并证明其具有上级抗菌活性。然而，其抗菌机制仍不明确。本研究旨在阐明多肽F1对金黄色葡萄球菌的抗菌机制。用化学法测定细胞内β-半乳糖苷酶漏出量，原子吸收光谱法测定钾离子释放量，透射电镜观察细胞形态学变化，电泳分析和原子力显微镜联合检测DNA结合活性等方法对F1肽的抗菌作用进行了表征。我们的研究结果表明，肽F1通过破坏细菌细胞膜和通过结合细胞质中的基因组DNA来发挥其杀菌作用，这两者都导致细胞快速死亡。(C)2015爱思唯尔有限公司版权所有。
Previously, we have isolated a novel bacteriocin, peptide Fl from Tibetan Kefir, and demonstrated its superior antimicrobial activity. However, its antimicrobial mechanism is still undefined. This study was aimed to elucidate the antimicrobial mechanism of peptide Fl against Staphylococcus aureus. The antimicrobial effects of peptide Fl were characterized by the following methods: chemical assay to quantify cytoplasmic fi-galactosidase leakage, atomic absorption spectrometry to measure the released potassium ions, transmission electron microscopy to visualize the cellular morphological changes, and electrophoresis analysis and atomic force microscopy together to exam the DNA binding activity. Our results revealed that peptide Fl exerted its bactericidal effects by damaging bacterial cell membranes and by binding to the genomic DNA in the cytoplasm, which both led to rapid cell death. (C) 2015 Elsevier Ltd. All rights reserved.