Evaluation of saliva as a source of human DNA for population and association studies
Evaluation of saliva as a source of human DNA for population and association studies
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DOI：
10.1016/j.ab.2006.03.021
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发表时间：
2006-06-15
影响因子：
2.9
通讯作者：
Nasidze, Ivan
中科院分区：
文献类型：
--
作者：
Quinque, Dominique;Kittler, Ralf;Nasidze, Ivan
开发了一种简单的非侵入性唾液样本采集和 DNA 提取程序。平均而言，人类 DNA 量（通过基于 TaqMan 的测定法测量）约为 11.4 pg/mL 唾液，这比从其他非侵入性样本（例如脸颊拭子）中获得的要多。然而，唾液样本中存在大量非人类 DNA（高达总提取 DNA 的 90%），因此需要专门针对人类 DNA 的 DNA 定量方法。我们能够从 37°C 保存长达 30 天的唾液样本中可靠、准确地分型不同的遗传标记（mDNA 序列、Y 染色体单核苷酸多态性和胞质微卫星位点），使该方法非常适合现场条件并方便将样本运回实验室。因此，唾液可以被认为是多种遗传学研究的可靠 DNA 来源。 (c) 2006 Elsevier Inc. 保留所有权利。
A simple noninvasive procedure for saliva sample collection and DNA extraction was developed. On average, the amount of human DNA (as measured by a TaqMan-based assay) was about 11.4 pg/mL saliva, which is more than can be obtained from other noninvasive samples such as cheek swabs. However, the presence of large amounts of nonhuman DNA (up to 90% of the total extracted DNA) in saliva samples does necessitate DNA quantitation methods that are specific for human DNA. We were able to reliably and accurately type different genetic markers (mDNA sequences, Y-chromosomal single-nucleotide polymorphisms, and amosomal microsatellite loci) from saliva samples stored for up to 30 days at 37 C, making this method well-suited for field conditions and convenient transportation of samples back to the laboratory. Thus, saliva can be considered a reliable source of DNA for a wide variety of genetic studies. (c) 2006 Elsevier Inc. All rights reserved.