Connexin 26 induces growth suppression, apoptosis and increased efficacy of doxorubicin in prostate cancer cells.
Connexin 26 induces growth suppression, apoptosis and increased efficacy of doxorubicin in prostate cancer cells.
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DOI：
10.3892/or.11.2.537
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发表时间：
2004-02
期刊：
影响因子：
4.2
通讯作者：
Motoyoshi Tanaka;H. Grossman
中科院分区：
文献类型：
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作者：
Motoyoshi Tanaka;H. Grossman
连接蛋白26（Cx 26）编码一种间隙连接蛋白，是一种公认的肿瘤抑制基因。我们评估了Cx 26强制表达对三种人前列腺癌细胞系PC-3、LNCap和DU-145的影响。用Cx 26腺病毒载体（Ad-Cx 26）或对照载体感染这三种细胞系或模拟感染。我们检测了细胞生长、细胞周期、细胞凋亡以及与阿霉素联合治疗的疗效。与对照组相比，Ad-Cx 26感染抑制了所有细胞系的生长，并诱导细胞周期停滞在G2/M期和凋亡。Ad-Cx 26可降低Bcl-2的表达。LNCaP细胞的生长被单独的Ad-Cx 26显著抑制。与单独使用Ad-Cx 26或阿霉素相比，联合基因治疗和化疗对PC-3和DU-145具有更大的生长抑制作用。Cx 26的强制表达抑制前列腺癌细胞的生长并降低Bcl-2的表达。联合Cx 26基因治疗与阿霉素导致更大的生长抑制。
Connexin 26 (Cx26) encodes a gap junction protein and is a putative tumor suppressor gene. We evaluated the effect of forced expression of Cx26 on three human prostate cancer cell lines, PC-3, LNCap, and DU-145. The three cell lines were infected with a Cx26 adenovirus vector (Ad-Cx26) or a control vector or were mock infected. We tested cell growth, cell cycle, apoptosis, and the efficacy of combined treatment with doxorubicin. Ad-Cx26 infection suppressed the growth of all the cell lines compared with controls and induced cell cycle arrest at the G2/M phase and apoptosis. Ad-Cx26 decreased the expression of Bcl-2. LNCaP cell growth was dramatically suppressed by Ad-Cx26 alone. PC-3 and DU-145 had greater growth suppression with combined gene therapy and chemotherapy than with either Ad-Cx26 or doxorubicin alone. Forced expression of Cx26 suppresses the growth of prostate cancer cells and decreases the expression of Bcl-2. Combining Cx26 gene therapy with doxorubicin results in greater growth suppression.