Calcofluor and ink-potassium hydroxide preparations for identifying fungi.
Calcofluor and ink-potassium hydroxide preparations for identifying fungi.
复制标题
用于识别真菌的卡尔科弗卢和墨水-氢氧化钾制剂。
DOI：
10.1016/0002-9394(85)90629-4
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发表时间：
1985
影响因子：
4.2
通讯作者：
Kaufman,HE
中科院分区：
文献类型：
--
作者：
Arffa,RC;Avni,I;Ishibashi,Y;Robin,J;Kaufman,HE
在兔角膜真菌感染的刮片、活检标本和组织切片中，鉴定出茄病镰刀菌、烟曲霉菌和白色念珠菌这三种最常见的眼部真菌病原体。这些染色还在四名角膜真菌病患者的样本中鉴定出真菌生物。这两种程序都不需要超过几分钟的时间来执行，也不需要大量的培训或经验来翻译。用钙氟染色的标本可以立即检测，但可能不能识别所有真菌。更灵敏的墨水-氢氧化钾制剂应在18至24小时后进行检查，与钙氟化法相比，提供假阳性结果的可能性较小。
Calcofluor and ink-potassium hydroxide preparations identifiedFusarium solani, Aspergillus fumigatus, andCandida albicans, the three most common ocular fungal pathogens, in scrapings, biopsy specimens, and tissue sections of corneal mycotic infections in rabbits. These stains also identified fungal organisms in specimens from four human patients with keratomycoses. Neither procedure requires more than a few minutes to perform or extensive training or experience to interpret. The specimen stained with calcofluor can be examined immediately, but may not identify all fungi. The more sensitive ink-potassium hydroxide preparation should be examined after 18 to 24 hours, and is less likely to provide false-positive results than the calcofluor method.