Moderate intensity of regular exercise improves cardiac SR Ca2+ uptake activity in ovariectomized rats
Moderate intensity of regular exercise improves cardiac SR Ca2+ uptake activity in ovariectomized rats
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DOI：
10.1152/japplphysiol.00407.2009
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发表时间：
2009-10-01
影响因子：
3.3
通讯作者：
Wattanapermpool, Jonggonnee
中科院分区：
文献类型：
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作者：
Bupha-Intr, Tepmanas;Laosiripisan, Jitanan;Wattanapermpool, Jonggonnee
Bupha-Intr T，Laosiripisan J，Wattanaperpool J.中等强度的定期运动改善卵巢切除大鼠心脏SR Ca 2+摄取活性。J Appl Physiol 107：1105-1112，2009.首次发表于2009年8月13日; doi：10.1152/japplphysiol.00407.2009。通过测定去卵巢大鼠9周中等强度跑台运动后肌浆网（SR）细胞内Ca 2+清除活性的变化，评价规律运动对保护雌性激素剥夺所致心脏恶化结果的影响。尽管诱导心脏肥大的运动组的假手术和卵巢切除大鼠，运动训练没有影响SR Ca 2+摄取和SR Ca 2 +-ATP酶（SERCA）在激素完整的大鼠心脏。然而，运动训练正常化抑制最大SR钙摄取和SERCA活性在卵巢切除大鼠心脏。虽然运动训练使卵巢切除大鼠pCa（-log[Ca 2 +]）-SR Ca 2+摄取关系的快速变化正常化，但在运动的假手术大鼠中未检测到任何影响。在SERCA和受磷蛋白（PLB）磷酸化水平上也观察到类似的现象;在卵巢切除大鼠中，运动训练增强SERCA表达，达到假手术大鼠的水平，其中久坐组和运动组之间的SERCA和磷酸化PLB水平没有差异。此外，运动训练可消除去卵巢大鼠心肌中磷酸化Thr（17）PLB的减少。这些结果表明，在正常生理条件下，定期运动维持心脏SR Ca 2+摄取的分子激活，并且能够诱导对卵巢性剥夺状态下心脏SR Ca 2+摄取的保护性影响。
Bupha-Intr T, Laosiripisan J, Wattanapermpool J. Moderate intensity of regular exercise improves cardiac SR Ca2+ uptake activity in ovariectomized rats. J Appl Physiol 107: 1105-1112, 2009. First published August 13, 2009; doi:10.1152/japplphysiol.00407.2009.-The impact of regular exercise in protecting cardiac deteriorating results of female sex hormone deprivation was evaluated by measuring changes in intracellular Ca2+ removal activity of sarcoplasmic reticulum (SR) in ovariectomized rats following 9-wk treadmill running exercise at moderate intensity. Despite induction of cardiac hypertrophy in exercised groups of both sham-operated and ovariectomized rats, exercise training had no effect on SR Ca2+ uptake and SR Ca2+-ATPase (SERCA) in hormone intact rat heart. However, exercise training normalized the suppressed maximum SR Ca2+ uptake and SERCA activity in ovariectomized rat heart. While exercise training normalized the leftward shift in pCa (-log[Ca2+])-SR Ca2+ uptake relation in ovariectomized rats, no effect was detected in exercised sham-operated rats. Similar phenomena were also observed on SERCA and on phospholamban (PLB) phosphorylation levels; exercise training in ovariectomized rats enhanced SERCA expression to reach the level as that in sham-operated rats, in which there were no differences in SERCA and phospho-PLB levels between sedentary and exercised groups. In addition, the reduction in phospho-Thr(17) PLB in myocardium of ovariectomized rats was abolished by exercise training. These results showed that regular exercise maintains the molecular activation of cardiac SR Ca2+ uptake under normal physiological conditions and is able to induce a protective impact on cardiac SR Ca2+ uptake in ovarian sex hormone-deprived status.