Degradation rate of DNA scaffolds and bone regeneration
Degradation rate of DNA scaffolds and bone regeneration
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DOI：
10.1002/jbm.b.34102
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发表时间：
2019-01-01
影响因子：
3.4
通讯作者：
Ohno, Jun
中科院分区：
文献类型：
--
作者：
Matsumoto, Ayako;Kajiya, Hiroshi;Ohno, Jun
植入骨缺损部位的支架必须达到最佳的生物降解率，同时随着新骨形成的进展适当地填充空隙。我们最近开发了一种独特的由鲑鱼脱氧核糖核酸（DNA）和鱼精蛋白组成的生物材料，可作为组织工程的骨传导支架。本研究的目的是阐明支架的降解率如何影响骨再生。我们采用大鼠皮肤侧腹皮下模型和大鼠颅骨缺损模型研究了鲑鱼DNA支架降解率与新骨形成的关系。支架的降解速率与紫外光照射预处理时间成正比。在皮下组织植入试验中，支架的生物降解率也取决于紫外线照射的持续时间。与照射0或3小时的支架相比，照射0.5小时的支架保持了磷酸盐的逐渐生物降解。在颅骨缺损模型中，我们发现，与照射0或3.0小时的支架相比，照射0.5小时的支架处理大鼠的新骨形成更高。目前的结果表明，生物降解支架的生物工程对骨再生很重要。(c) 2018 Wiley期刊公司[J]中国生物医学工程学报，2016,31(2):557 - 557。
Scaffolds implanted into bone defect sites must achieve optimal biodegradation rates while appropriately filling the void as new bone formation progresses. We recently developed a unique biomaterial consisting of salmon deoxyribose nucleic acid (DNA) and protamine, which can be used as an osteoconductive scaffold for tissue engineering. The aim of the present study was to elucidate how the degradation rate of the scaffold affects bone regeneration. We examined the relationships between the degradation rate of salmon DNA scaffolds and new bone formation using a rat skin flank subcutaneous model and rat calvarial defect model. The degradation rates of the scaffolds were proportional to the durations of pretreatment with ultraviolet (UV) light irradiation. The biodegradation rates of the scaffolds were also dependent on the duration of UV irradiation, as tested a subcutaneous tissue implantation. Scaffolds irradiated with UV light for 0.5 h maintained gradual biodegradation of phosphate compared with scaffolds irradiated for 0 or 3 h. In the calvarial defect model, we found that new bone formation was higher in rats treated with scaffolds irradiated with UV light for 0.5 h compared with those irradiated with UV light for 0 or 3.0 h. The present results suggest that bioengineering of scaffolds for biodegradation is important to regenerate bone. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 122-128, 2019.