Application of manure from cattle administered antibiotics has sustained multi-year impacts on soil resistome and microbial community structure
Application of manure from cattle administered antibiotics has sustained multi-year impacts on soil resistome and microbial community structure
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DOI：
10.1016/j.soilbio.2021.108252
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发表时间：
2021-04
影响因子：
9.7
通讯作者：
Sarah Shawver;Carl Wepking;S. Ishii;M. Strickland;B. Badgley
中科院分区：
文献类型：
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作者：
Sarah Shawver;Carl Wepking;S. Ishii;M. Strickland;B. Badgley
在农业生态系统中，施用经抗生素处理的牲畜粪便有可能将抗生素化合物、耐药细菌和抗生素耐药基因（ARG）传播到土壤中。尽管抗生素耐药性的环境传播是一个主要的人类健康问题，但很少有研究关注施用抗生素的牲畜粪便对土壤微生物群落的长期影响。我们研究了三年的重复粪便添加不同的抗生素治疗下的微生物群落结构和ARG丰度的牛的影响。虽然粪肥的添加改变了土壤细菌和真菌群落，从牛施用抗生素的粪肥进一步改变土壤细菌群落，但不是真菌，相比粪肥无抗生素牛。此外，除了粪便从无寄生虫牛导致几个ARG的丰度增加与土壤相比，没有粪便投入，但粪便从牛管理的抗生素没有改变ARG丰度的整体配置文件相比，粪便从无寄生虫牛。最后，虽然细菌和真菌群落结构和ARG丰度各不相同的年份，粪肥处理的影响，在整个三年期间是持久的。总之，我们的研究结果表明，粪肥和抗生素对土壤微生物群落的影响可以持续很长一段时间的重复施肥。此外，解决抗生素耐药性危机的土壤管理战略应考虑粪肥管理的更广泛背景。
In agroecosystems, application of manure from livestock treated with antibiotics has the potential to spread antibiotic compounds, resistant bacteria, and antibiotic resistance genes (ARGs) to soil. Although environmental transmission of antibiotic resistance is a major human health concern, few studies have looked at long-term effects on soil microbial communities from applying manure from livestock administered antibiotics. We examined the impacts of three years of repeated manure additions from cattle under different antibiotic treatments on microbial community structure and ARG abundances. While manure additions altered both soil bacterial and fungal communities, manure from cattle administered antibiotics further altered soil bacterial communities, but not fungal, compared to manure from antibiotic-free cattle. Furthermore, addition of manure from antibiotic-free cattle resulted in increased abundances of several ARGs compared to soil with no manure inputs, but manure from cattle administered antibiotics did not change overall profiles of ARG abundances compared to manure from antibiotic-free cattle. Finally, although bacterial and fungal community structure and ARG abundances varied among years, manure treatment effects on each were persistent during the full three-year period. Taken together, our results suggest that manure and antibiotic impacts on soil microbial communities can persist for long periods of repeated manure application. Furthermore, soil management strategies for addressing the antibiotic resistance crisis should consider the broader context of manure management.