Secretion of β-1,3-glucanases by the yeast Pichia membranifaciens and its possible role in the biocontrol of Botrytis cinerea causing grey mold disease of the grapevine
Secretion of β-1,3-glucanases by the yeast Pichia membranifaciens and its possible role in the biocontrol of Botrytis cinerea causing grey mold disease of the grapevine
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DOI：
10.1007/s00284-001-0011-y
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发表时间：
2002-06-01
影响因子：
2.6
通讯作者：
Paul, B
中科院分区：
文献类型：
--
作者：
Masih, EI;Paul, B
从葡萄皮上分离到一株膜质毕赤酵母FY-101菌株，该菌株对葡萄藤灰霉病具有拮抗作用。当在固体和液体培养基中一起生长时，酵母菌会抑制灰葡萄孢，而灰葡萄孢又会失去在葡萄藤幼苗上产生灰霉病症状的能力。β-1，3-葡聚糖酶的分泌是与这种拮抗作用相关的可能机制之一。体外实验证实该酵母可用作抗B的生物控制生物。灰叶这里给出了这种拮抗作用和P. membranifaciens产生β-1，3-葡聚糖酶的说明。
Pichia membranifaciens strain FY-101, isolated from grape skin, was found to be antagonistic to Botrytis cinerea, the causal organism of the grey mold disease of the grapevine. When grown together on solid as well as in liquid media, the yeast brings about the inhibition of Botrytis cinerea, which in turn loses its ability to produce the grey mold symptoms on the grapevine plantlets. The secretion of beta-1,3-glucanases by P. membranifaciens is one of the possible mechanisms related to this antagonism. In vitro experiments confirm that this yeast can be used as a biological control organism against B. cinerea. An account of this antagonism and the production of beta-1,3-glucanases by P. membranifaciens is given here.