Transcriptome dynamics of the stomatal lineage: birth, amplification, and termination of a self-renewing population.
Transcriptome dynamics of the stomatal lineage: birth, amplification, and termination of a self-renewing population.
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DOI：
10.1016/j.devcel.2015.01.025
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发表时间：
2015-04-06
影响因子：
11.8
通讯作者：
Bergmann DC
中科院分区：
文献类型：
--
作者：
Adrian J;Chang J;Ballenger CE;Bargmann BO;Alassimone J;Davies KA;Lau OS;Matos JL;Hachez C;Lanctot A;Vatén A;Birnbaum KD;Bergmann DC
发育转变可以用形态学和单个基因的作用来描述，也可以用全局转录和表观遗传变化来描述。然而，对于完整的发育组织，转录组变化的时间解剖是罕见的。在拟南芥叶片成体干细胞谱系发育过程中，我们使用RNA测序和微阵列平台来量化荧光活化细胞分选分离的标记细胞的基因表达，以生成细胞类型特异性转录组。我们展示了这种早期谱系连接细胞类型的调控模块，这些细胞类型以前被认为是在单独的控制下，并为不同发育决定的基因家族个体成员的招募提供了证据。由于气孔具有重要的生理意义，并且气孔系细胞表现出典型的分裂、细胞命运和细胞信号行为，因此该数据集为进一步研究基本发育过程提供了宝贵的资源。
Developmental transitions can be described in terms of morphology and the roles of individual genes, but also in terms of global transcriptional and epigenetic changes. Temporal dissections of transcriptome changes, however, are rare for intact, developing tissues. We used RNA sequencing and microarray platforms to quantify gene expression from labeled cells isolated by Fluorescence Activated Cell Sorting to generate cell-type specific transcriptomes during development of an adult stem-cell lineage in the Arabidopsis leaf. We show regulatory modules in this early lineage link cell types that had previously been considered to be under separate control and provide evidence for recruitment of individual members of gene families for different developmental decisions. Because stomata are physiologically important and because stomatal lineage cells exhibit exemplary division, cell fate and cell signaling behaviors, this dataset serves as a valuable resource for further investigations of fundamental developmental processes.