Pap1p-dependent upregulation of thioredoxin 3 and thioredoxin reductase genes from the fission yeast under nitrosative stress.
Pap1p-dependent upregulation of thioredoxin 3 and thioredoxin reductase genes from the fission yeast under nitrosative stress.
复制标题
亚硝化胁迫下裂殖酵母中硫氧还蛋白 3 和硫氧还蛋白还原酶基因的 Pap1p 依赖性上调。
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
C. Lim
中科院分区：
文献类型：
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作者：
Minsik Park;Hyeon;A. Park;Kisup Ahn;Hye;C. Lim
硫氧还蛋白系统由硫氧还蛋白、硫氧还蛋白还原酶和NADPH组成，参与对各种胁迫的反应。在裂殖酵母中，TRX3(+)和TrxR(+)基因分别编码硫氧还蛋白3和硫氧还蛋白还原酶。利用LacZ融合基因研究了它们的转录调控。在Pap1p阳性细胞中，一氧化氮生成的硝普钠显著促进Trx3(+)-lacZ融合基因合成β-半乳糖苷酶，而在Pap1p阴性细胞中则不明显。同样，TrxR(+)-lacZ融合基因合成β-半乳糖苷酶也被硝普钠以Pap1p依赖的方式上调。在Pap1p阳性的细胞中，S-亚硝基谷胱甘肽也能促进Trx3(+)-lacZ和TrxR(+)-lacZ融合基因合成β-半乳糖苷酶，而对Pap1p阴性的细胞则没有促进作用。总之，编码硫氧还蛋白3和硫氧还蛋白还原酶的基因在亚硝化胁迫下以Pap1p依赖的方式上调。
The thioredoxin system, consisting of thioredoxin, thioredoxin reductase, and NADPH, is involved in the response against a variety of stresses. The TRX3(+) and TrxR(+) genes encode thioredoxin 3 and thioredoxin reductase, respectively, in the fission yeast Schizosaccharomyces pombe . Their transcriptional regulations were studied using the lacZ fusion genes. Synthesis of β-galactosidase from the TRX3(+)-lacZ fusion gene was markedly enhanced by nitric-oxide-generating sodium nitroprusside in the Pap1p-positive cells but not in the Pap1p-negative cells. Similarly, synthesis of β-galactosidase from the TrxR(+)-lacZ fusion gene was upregulated by sodium nitroprusside in a Pap1p-dependent manner. Synthesis of β-galactosidase from the TRX3(+)-lacZ and TrxR(+)-lacZ fusion genes was also enhanced by S-nitrosoglutathione in the Pap1p-positive cells but not in the Pap1p-negative cells. In brief, the S. pombe genes encoding thioredoxin 3 and thioredoxin reductase are upregulated under nitrosative stress in a Pap1p-dependent manner.
影响因子：
3.3
作者：
Hromatka, BS;Noble, SM;Johnson, AD
通讯作者：
Johnson, AD