Characterization of catalase and superoxide dismutase in Staphylococcus carnosus 833 strain
Characterization of catalase and superoxide dismutase in Staphylococcus carnosus 833 strain
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DOI：
10.1046/j.1365-2672.2001.01411.x
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发表时间：
2001-09-01
影响因子：
4
通讯作者：
Talon, R
中科院分区：
文献类型：
--
作者：
Barrière, C;Leroy-Sétrin, S;Talon, R
目的：肉糖葡萄球菌用作发酵肉制品的发酵剂，可降低脂肪氧化产生的挥发物水平。方法和结果：用分光光度法测定过氧化氢酶和超氧化物歧化酶的活性，并将其显示在非变性的聚丙烯酰胺凝胶上。通过聚合酶链式反应鉴定出相应的超氧化物歧化酶基因。Southern杂交和酶学分析表明，葡萄球菌中含有单一的过氧化氢酶和单一的超氧化物歧化酶。肉糖833菌株。编码葡萄球菌的基因。肉糖超氧化物歧化酶编码一种与需要锰的超氧化物歧化酶密切相关的蛋白质。过氧化氢酶和超氧化物歧化酶水平在对数中期升高。只有过氧化氢酶能被氧、硝酸盐或亚硝酸盐诱导，而葡萄糖不能诱导。金属离子限制可提高过氧化氢酶活性，降低超氧化物歧化酶活性。肉糖在香肠生产中遇到的条件下合成这两种酶。这些结果可以解释葡萄球菌的抗氧化特性。肉糖发酵剂培养。这项研究的意义和影响：了解葡萄球菌的抗氧化特性将允许在肉类发酵产品中更合理地使用这些发酵剂。
Aims: Staphylococcus carnosus, used as starter culture in fermented meat products, decreases the level of volatiles arising from lipid oxidation. To analyse its antioxidant capacities, catalase and superoxide dismutase (SOD) were characterized.Methods and Results: Catalase and SOD activities were measured with spectrophotometric methods and visualized on non-denaturing polyacrylamide gels. The corresponding sod gene was identified by PCR. Southern hybridizations and enzymatic analyses showed that there was a single catalase and a single SOD in Staph. carnosus 833 strain. The gene encoding the Staph. carnosus SOD was found to encode a protein closely related to SOD requiring manganese. Catalase and SOD levels increased in mid-log phase. Only catalase was induced by oxygen, nitrate or nitrite while glucose induced neither enzyme. Metal ion limitation increased catalase and decreased SOD activities.Conclusions: Staph. carnosus synthesizes both enzymes in conditions encountered in sausage manufacturing. These results could explain the antioxidant properties of Staph. carnosus starter culture.Significance and Impact of the Study: The knowledge of the antioxidant properties of Staphylococci will allow a more rational use of these starters in meat fermented products.