Living Single Cell Analysis Platform Utilizing Microchannel, Single Cell Chamber, and Extended-nano Channel
Living Single Cell Analysis Platform Utilizing Microchannel, Single Cell Chamber, and Extended-nano Channel
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DOI：
10.2116/analsci.32.75
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发表时间：
2016-01-01
影响因子：
1.6
通讯作者：
Kitamori, Takehiko
中科院分区：
文献类型：
--
作者：
Lin, Ling;Mawatari, Kazuma;Kitamori, Takehiko
近年来，单细胞分析引起了人们极大的兴趣。特别是实现活体单细胞分析是研究单细胞动态过程的最终目标。然而，单细胞体积为pL规模，即使通过微流控技术（nL-sub nL）也很难实现活体单细胞分析。在此，通过集成单细胞室和扩展纳米通道（aL-fL体积）开发了一种新型微流体平台。通过压力驱动流量控制分离并培养单个细胞12小时以上。此外，还开发了一种电阻测量方法来监测细胞活力，无需荧光标记。该平台将利用扩展纳米空间的新颖分析功能，为活体单细胞分析提供一种新方法。
Single cell analysis has been of great interest in recent years. In particular, to achieve living single cell analysis is the ultimate goal to study the dynamic process of the single cell. However, single cell volume is pL in scale, and it is difficult to realize living single cell analysis, even by microfluidic technology (nL-sub nL). Herein, a novel microfluidic platform was developed by integrating a single cell chamber and an extended-nano channel (aL-fL volume). A single cell was isolated and cultured for more than 12 h by pressure-driven flow control. In addition, an electric resistance measurement method was developed to monitor the cell viability without fluorescence labeling. This platform will provide a new method for living single cell analysis by utilizing the novel analytical functions of the extended-nano space.