Transposable elements contribute to the genome plasticity of Ralstonia solanacearum species complex
Transposable elements contribute to the genome plasticity of Ralstonia solanacearum species complex
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DOI：
10.1099/mgen.0.000374
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发表时间：
2020-05-01
影响因子：
3.9
通讯作者：
Santana, Mateus Ferreira
中科院分区：
文献类型：
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作者：
Goncalves, Osiel Silva;Campos, Kiara Franca;Santana, Mateus Ferreira
青枯雷尔氏菌（Ralstonia solanacearum）是一种严重的土传植物病原菌，其广泛的遗传多样性导致了R。青枯菌（R.青枯菌物种复合体（RSSC）]。插入序列（IS）被认为在该病原体的基因组进化中起着重要作用。在这里，我们确定和分析转座因子（TE），IS和转座子，在106 RSSC基因组和15罗尔斯通氏菌属。我们在62个代表性RSSC菌株和密切相关的Ralstonia spp的全基因组中定位了10 259个IS元件。一组独特的20个IS家族在菌株中广泛分布，IS5和IS3是最丰富的。我们的研究结果表明，六个新的转座子序列属于Tn3家族携带乘客基因编码抗生素抗性和无毒蛋白。此外，在假青枯雷尔氏菌菌株中证实了与IS相关的内部重排事件。我们还定位了干扰无毒基因的IS元件，这为IS在RSSC毒力进化中起重要作用提供了证据。此外，通过转录组分析和DNA杂交，证明了在R。青枯菌分离物总之，我们提供了RSSC基因组中TE的集体数据，为了解它们对这种重要植物病原体的基因组进化和多样性的进化影响开辟了新的途径。
The extensive genetic diversity of Ralstonia solanacearum, a serious soil-borne phytopathogen, has led to the concept that R. solanacearum encompasses a species complex [R. solanacearum species complex (RSSC)]. Insertion sequences (ISs) are suggested to play an important role in the genome evolution of this pathogen. Here, we identified and analysed transposable elements (TEs), ISs and transposons, in 106 RSSC genomes and 15 Ralstonia spp. We mapped 10 259 IS elements in the complete genome of 62 representative RSSC strains and closely related Ralstonia spp. A unique set of 20 IS families was widespread across the strains, IS5 and IS3 being the most abundant. Our results showed six novel transposon sequences belonging to the Tn3 family carrying passenger genes encoding antibiotic resistance and avirulence proteins. In addition, internal rearrangement events associated with ISs were demonstrated in Ralstonia pseudosolanacearum strains. We also mapped IS elements interrupting avirulence genes, which provided evidence that ISs plays an important role in virulence evolution of RSSC. Additionally, the activity of ISs was demonstrated by transcriptome analysis and DNA hybridization in R. solanacearum isolates. Altogether, we have provided collective data of TEs in RSSC genomes, opening a new path for understanding their evolutionary impact on the genome evolution and diversity of this important plant pathogen.