Effect of pigment packaging on diffuse reflectance spectroscopy of samples containing red blood cells
Effect of pigment packaging on diffuse reflectance spectroscopy of samples containing red blood cells
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DOI：
10.1364/ol.29.000965
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发表时间：
2004-05-01
期刊：
影响因子：
3.6
通讯作者：
Foster, TH
中科院分区：
文献类型：
--
作者：
Finlay, JC;Foster, TH
我们提出了漫反射测量的结果，使表面上的组织模拟幻影含有完整的人红细胞。这些测量结果表明，血红蛋白在自然环境中的吸收光谱与均匀流体溶液中的吸收光谱有明显的不同，特别是在吸收最高的光谱区域。我们表明，这种差异可以用Duysens [Biochim]开发的色素包装理论来解释。Biophys。Acta 19, 1(1956)]，采用考虑了这一效应的基谱提高了漫反射光谱拟合的精度。(C) 2004美国光学学会。
We present the results of diffuse reflectance measurements made on the surface of a tissue-simulating phantom containing intact human erythrocytes. These measurements indicate that the absorption spectrum of hemoglobin in its natural environment is significantly different from that measured in homogeneous fluid solution, especially in the spectral regions of highest absorption. We show that this difference can be explained by the pigment packaging theory developed by Duysens [Biochim. Biophys. Acta 19, 1 (1956)] and that the adoption of basis spectra that take this effect into account improves the accuracy of fitting diffuse reflectance spectra. (C) 2004 Optical Society of America.