Polysaccharides of Brassica rapa L. attenuate tumor growth via shifting macrophages to M1-like phenotype
Polysaccharides of Brassica rapa L. attenuate tumor growth via shifting macrophages to M1-like phenotype
复制标题
白菜多糖通过将巨噬细胞转变为 M1 样表型来减弱肿瘤生长
DOI：
10.1002/ptr.7545
复制
发表时间：
2022-06-29
影响因子：
7.2
通讯作者：
Bai, Liping
中科院分区：
文献类型：
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作者：
Guo, Weiwei;Liu, Xiujun;Bai, Liping
巨噬细胞是主要的肿瘤浸润性白细胞，并且肿瘤相关巨噬细胞（TAM）在癌症相关炎症中起关键作用，因为它们显示出与M1（肿瘤抑制性巨噬细胞）或M2（肿瘤促进性巨噬细胞）表型的交替极化。恰玛（B. rapa）具有抗肿瘤和增强免疫力的作用，而B.已报道芜菁（BRP）对巨噬细胞具有免疫调节作用。本研究以巨噬细胞极化为研究对象，探讨BRP体内、外抗肿瘤作用的机制。结果表明，BRP可促进M1标记物的表达，包括iNOS、考克斯-2、HLA-DR、CD 11b和M1相关细胞因子。BRP处理抑制IL-4诱导的M2标志物Arg-1、CD 206和CD 163的表达，导致体内和体外共培养实验中肿瘤生长的抑制。BRP对STAT信号通路的激活有显著的调节作用，是TAM极化的重要信号。总之，结果表明BRP通过介导巨噬细胞极化而具有抗肿瘤作用。
Macrophages are the major tumor-infiltrating leukocytes, and tumor-associated macrophages (TAM) play a critical role in cancer-related inflammation since they show alternative polarization to M1 (tumor-inhibited macrophages) or M2 (tumor-promoted macrophages) phenotype. Brassica rapa L. (B. rapa) has been clinically proven to have anti-tumor and immunity-enhancing activity, and the polysaccharides of B. rapa (BRP) have been reported to have an immunoregulatory effect on macrophages. In this study, we focus on macrophage polarization to investigate the mechanism of anti-tumor response of BRP in vivo and in vitro. We found that BRP improved the expression of M1 markers, including iNOS, COX-2, HLA-DR, CD11b and M1-related cytokines. The expression of M2 markers Arg-1, CD206 and CD163 induced by IL-4 were inhibited by BRP treatment, resulting in the inhibition of tumor growth both in vivo and in co-culture experiments in vitro. The activation of STAT signaling pathway were significantly regulated by BRP, which are important signals in TAM polarization. Overall, the results indicated that BRP has anti-tumor effect through mediating macrophage polarization.